Hi all,
I need expert opinions on this matter as my plant samples are detached during washing step of FISH. I used agarose as well as transparent nail polish for this purpose and to me agarose didn't work at all but nail polish works slightly. It provides a base to sliced plant tissue, when I put my hybridization slide into the washing buffer (50 ml falcon) and then in water bath; after heating up to 48 degree Celsius, the nail polish is no more stick and my plant samples swim in freely in the media. Though I doubt that it may not affect the hybridization but still I am not sure. Can you please share your practical experiences?
Thanks in advance
Hi
Many useful references on attachment 1
https://www.sciencedirect.com/science/article/pii/S0949265817300350
2
https://www.tandfonline.com/doi/full/10.1080/21622515.2013.770564?src=recsys
4
https://pediatrics.aappublications.org/content/pediatrics/93/5/826.full.pdf
8
https://books.google.iq/books/about/In_Situ_Hybridization.html?id=HV7gjZlMGHUC&redir_esc=y
9
http://www.nature.com/modpathol/journal/v16/n9/full/3880868a.html?foxtrotcallback=true
10
https://academic.oup.com/biohorizons/article/3/1/85/228880/Applications-of-fluorescence-in-situ-hybridization
14
https://www.slideshare.net/PatHeslopHarrison/in-situ-hybridization-methods-and-techniques-course-slides-pat-heslopharrison
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