I am trying to deplete rubisco from total protein extract from rice leave,I have solubilised some samples in 8M urea and others are in pellet form. I have tried to do with SepproÒ Rubisco Spin Columns,but it is mentioned that column is not compatible with urea . so if I will do buffer exchange protein concentration will loss and for another samples which are in not solubilised yet, I don't know how to proceed. I have also learnt about PEG fractionation ,I am really confused that which method should be use without the loss of protein.
Hi,
I think that there are antibodies and even commercial resins with these antibodies to pass your protein pellet (after resolubilization) over the column/ resin to extract Rubisco.
Alternatively, depending on your aims and size of your protein(s) of interest, any separation based on size factionation could be used.
Best wishes, good luck,
Tony Schaeffner
Munich, Germany
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