Hello everyone
I want to perform a prelimainary experiment for checking the resistance of six wheat varieties against wheat aphid under laboratory condition. For this I will sow 10 wheat seeds of every variety in separate pots. I will release 10 winged and 10 wingless adults aphids in each pot. After 10 and 20 days of releasing aphids, I will check the number of numphs, number of winged and wingless aphids and weight of aphids. I will use only one replication because of preliminary experiment.
I am confused how I will take data because of many aphids in one pot. Kindly suggest me about data collection.
immobilise/anaesthetise the aphids and count winged/wingless ones. Determine number of germinated seeds of wheat. You need up to 2/3 replicates for preliminary reliable results. It can be tedious, though. Good luck.
If you want to analyse your data it will not possible or not acceptable for researcher, because, with one replication may be you will find the percentage or relative abundance at least 3 replication you must do. Secondly for each variety you must collect data randomly according your objective per leave or stem etc...... wish you all the best
Hi Bilal,
Would be better if you specify which aphid species you want to work with. And also you need to be sure what aspect of resistance you want to measure (antibiosis, antixenosis or tolerance).
As a general procedure for antibiosis, you can quantify the intrinsic rate of increase (rm), or the mean relative growth rate (mrgr). You calculate these two in single seedlings, and MRGR may be the fastest approach and good approximation to rm.
If you want to measure tolerance, you can have a replicated trial, with two treatments (infested vs. non-infested), use single seedlings of about the same size, and compare biomass loss. This is may seem straight, but requires that you ensure similar conditions to both treatments, and same amount of aphids in each seedling. Depending of the aphid species you want to work, you can evaluate symptoms instead of biomass, for which you only need to infest individual seedlings with a determined amount of aphids and check back the symptoms after 15-20 days.
For antixenosis. Not many people measure this, unless they want to determine the main category of resistance that is operating in particular genotypes. This is because in practical terms, antixenosis is not very reliable in the field.
Cheers,
Leonardo
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Plant Biology
- Plant Biotechnology