By qualitative assay, we have found some bacteria showing positive to utilize nitrogen in Nfb media. Now we are trying to quantify nitrogen fixation by these endophytic bacteria. So if anyone can suggest which method we can apply to quantify nitrogen fixation . How is the Kjeldahl method?
You can go for quantitative estimation of nitrate reductase activity which may serve your purpose.
You could work with stable isotopes (15N2). Ohyama et al. did something similar in sugarcane plants.
Ohyama, T., Momose, A., Ohtake, N., Sueyoshi, K., Sato, T., Nakanishi, Y., & Ando, S. (2014). Nitrogen Fixation in Sugarcane. Advances in Biology and Ecology of Nitrogen Fixation, 47-70.
If you don't have access to a mass spectrometer I could imagine that an acetylene essay could work, too.
Hardy, R. W., Holsten, R. D., Jackson, E. K., & Burns, R. C. (1968). The acetylene-ethylene assay for N2 fixation: laboratory and field evaluation. Plant physiology, 43(8), 1185-1207.
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