MAP Tau, htau40, 2N4R, with 441 amino acids runs heavy on SDS-PAGE. What can explain a 22 kDa difference in actual vs apparent?

14 July 2023 1 4K Report

MAP Tau, htau40, 2N4R, has the actual weight of 45 kDa but runs as 67 kDa on SDS-PAGE. What can explain this much weight difference?

Is it specifically about Tau's unique structure effecting charge, or possible post translational modifications?

John Carter

I haven't worked with tau, but I know it contains lots of lysine. As SDS PAGE is based on movement of proteins due to negative charge, it seems possible the large positive charge may reduce migration.

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