Hello,

I usually use Iba1 (Wako) to measure microgliosis. You can also use GFAP to measure astrogliosis. In both cases, you should analyse integrated density or mean grey value of a specific ROI.

Hi Mickael Pereira

1. Microglia activation and Phagocytotic Property- IBA1+CD-68

2. Astrocyte activation- GFAP and Aquaporin-4

3. IL-1B, TNF-a, COX-2, IL-6 (Inflammatory markers released due to glial cells activation or neuroinflammation).

Thanks,

Hi Mickael Pereira, I usually use CD64'CD68'CD32 and CD11b to in both cases and agree with Louis-Chales Beland

Hello,

Thank you all for your responses.

It was a great help

Thank you again