Did someone used specific blocking antibodies that target the adhesion molecules involved in the interaction between hematopoietic cells and MSCs (specifically antibodies against VCAM-1 or α4β1 integrin) in in-vitro experiment?
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...
21 February 2021 8,659 1 View
The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...
20 February 2021 9,226 2 View
As a current undergraduate student majoring in Microbiology who is still learning DOE (Design of experiment) on my own, I have heard and read that an OFAT design is considered the most inefficient...
29 January 2021 8,256 3 View
After seeking for references, most of the solvents for Dess-Martin is DCM, but my compound has a poor solubility in DCM. Because my compound is acid-sensitive, I prefer not to use IBX to oxidize...
17 January 2021 3,586 3 View
I'm going to perform RNA-sequencing of the zebrafish brain with three different treatments, which are naive fish, alcohol withdrawal fish injected with Saline, alcohol withdrawal fish injected...
08 December 2020 4,643 2 View
I have 6 culture tank, 2 treatment with 3 replicate. I test temperature of every tank everyday, for 14 days. Now I want to compare whether this 2 treatment temperature have significant different...
06 December 2020 834 7 View
I downloaded the expression matrix data "GSE143416_Brain_data_TPM.txt.gz" of GSE143416 from Gene expression omnibus ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi). However, the first column of...
30 November 2020 1,304 3 View
Is there a reliable protocol to isolate and "purify" only tumorspheres in a sphere culture, that also contains some cell debris, some single cells that have failed to form spheres, and cell clumps...
17 November 2020 8,384 3 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
02 March 2021 10,003 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View