Need to obtain concentration of DNA/RNA?

Kiriakos Athanasiadis @Kiriakos-Athanasiadis

12 June 2024 1 711 Report

I need to use a photometer, so i have Cary Agilent and i would like to know what kind of cuvettes and its dimensions and volume i have to order. It's about RNA and DNA samples. Which are you recommended?

Gary James Hunter

check the manual for whichever spectrophotometer you have and the cuvette holders available. Not all "Cary Agilents" are the same (model name or number would have helped). Find the smallest cuvettes you can that are UV transparent. There are even some cuvettes that can turn your spec into a nanodrop (I have one).

Typically you should get down to 50 uL normal cuvettes and if you have the funding 3 uL for the nanodrop cuvette.

Badges
Science topic

More Kiriakos Athanasiadis's questions See All

I have some questions for calculation working with GC MS, help?

So, i m working during last month with GC-MS. When i'm making the extraction of samples e.g. PAH samples, i'm pipetting significant concentration of two internal controls into the solution. When i...

31 March 2024 6,550 1 View

How can i transform copies DNA to mg/kg?

I am working with an allergen and i am working using PCR, the result that offers the kit is copies DNA, although i need to give a result in mg/kg. Is any possible way? Thank you in advance,...

24 March 2024 5,656 3 View

Question about calculation for Standards GC-MS?

I have 4 substances for PAH, each one is 100ppm or 1000ppm, i would like to make a mix(4-PAH) of them of final volume 1mL and final concentration 100μg/kg. How can i proceed? Thank you in advance,

11 March 2024 5,658 2 View

Determination of Dioxins/Furans via GC-MS?

Hallo! I m working on GC-MS instrument these days and i am installing some methods. I would like to ask if you know any research paper or directly a method for Dioxins/Furans via GC-MS?

06 March 2024 7,842 2 View

I am using BIO0-RAD Tubes and Caps for my PCR Reactions, so when PCR starts the tubes are opened (95Celsius). What i have to do?

I am using PCR Instrument called SLAN from HONGSHI. Any clue?

23 January 2024 9,712 7 View

May i use knock out gene method to obtain confirmation for gene expression?

I would like to confirm PCR Results, so i thought that "knock out" of gene of interest will work. Who support this? If someone has different aspect would you please share?

17 January 2024 1,444 2 View

May i use Northern blot to ensure my RT-PCR results?

Is it highly recommended to use northern blot to ensure my RT-PCR results instead of LC-MS for example? Thank you in advance, Kiriakos

03 December 2023 1,794 2 View

What i have to pay attention in gene expression interpretation?

I m gonna make an excel for results interpretation for gene expression on food matrixes for quantification. How can i proceed? Thanks you in advance, Kiriakos

03 December 2023 5,813 0 View

How to convert Citric acid to Calcium citrate?

I have 10mg/kg obtained by using HPLC, How to convert to Calcium citrate? Thank you,

12 October 2023 4,330 3 View

PCR Technique for Dioxins/furans quantitative detection?

I am running via ELISA method Dioxins/furans analysis for food matrixes and i would like to ask if there is any PCR technique for quantitative determination. Thank you in advance,

11 October 2023 3,637 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,081 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,537 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,881 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,618 1 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,614 0 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 806 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,365 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 500 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 223 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,418 1 View