Hello everyone,

I am a student who could really benefit from someone else's opinion and experience. I've experienced poor 1kb + DNA (NEB) ladder formation on my agarose gel up to three times. They're all 1% gels (made with the same buffer as the running buffer) in TAE buffer with GelRed. I've experimented a bit with the voltage and duration of the run, but in all cases, I end up with a strange migration of the ladder. I also tried with a "fresh/new" ladder, but it didn't change anything.

Does anyone have an idea why this is happening and what could be causing it?