How to overcome the stickiness of smaller molecules/peptides to the plastic/PetriDish?

18 June 2024 1 6K Report

Use of excess amount of BSA is already tested but has not helped us. Addition of 0.01% Tween did not help us either.

Excess amount of BSA with respect to what? Defatted milk in PBS+tween 20 might help. If the molecules are small and have some hydrophobicity, they can still reach the surface. Better results should be obtained for larger molecules. Polyethylene glycol could also work, but still, better results would be obtained for larger molecules.

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