Does anyone know how to simulate the interaction between beam and solid elements?

I am trying to simulate the dynamic behavior of a pile group. In my model, pile and soil are modeled by beam and solid elements, respectively.

28 April 2014 134 2 View

What is the effect of ethanol contamination of RNA extraction in RT-qPCR? Can this be a reason for low amplification?

Cycle thresholds are about 25-29, and the product bands are weak on agarose gel electrophoresis.

03 June 2013 1,049 6 View

Protein 3D structure prediction.

I need to predict a protein's 3D structure. This protein has 0.22 identity with it's template. I used a lot of software for modeling (homology modeling & threading): MODELLER, phyre, ps2, esypred,...

12 February 2013 1,423 3 View

How to give 100 model from modeller?

I am working on a protein's 3D structure prediction and I want to use modeller. This program renders 5 model by defult. How can I give 100 model instead?

11 February 2013 2,484 2 View

Food granulation/ Maltodextrin granulation

How do I make food granules? Also; How to make granules with maltodextrin?

01 January 1970 2,175 3 View

Cheaper culture media instead of MRS for probiotic bacteria

What do you recommend cheaper culture media instead of MRS media for Lactobacillus bacteria ssp. plantarum?

01 January 1970 9,366 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

No title

23 February 2021 4,014 3 View