Have you ever seen a small number of cells, 50-100 thousand, after centrifugation as a dry pellet in an Eppendorf tube without a microscope? Are there methods to potentially stain the cells in the suspension so that they can be visible?
Good morning. I have recently switched computers and gotten a new edition of Graphpad. I am trying to open a very large file containing all of my graphs for a project, but am encountering an...
22 March 2020 6,810 1 View
I have an established cell line that expresses GFP constitutively. Once I transfect the cell line, there shouldn't any GFP expression since the DNA payload doesn't have a fluorescence marker. My...
23 September 2019 3,940 1 View
Good evening - I need to come up with a project idea for co-op. The project needs to be mostly FACS-related but also give exposure to other techniques, such as cell culture and molecular biology...
18 September 2019 8,911 2 View
Hi everyone! I was wondering if anyone might have any ideas of how to conduct some potential analyses for some research I am working on. I am interested in the moderating role of trait emotion...
09 May 2019 5,118 3 View
About connecting SAP with matlab
16 November 2018 2,663 3 View
I want to link matlab to opensees to complete the dynamic stability analysis of a 2D frame
13 August 2018 3,721 4 View
Dear all, Anybody could explain to me which method is better for measurement the level of toll like receptor protein in tumor tissue , Immunohistochemistry vs western blot? Thanks in advance.
09 April 2018 6,343 5 View
Dear all, For measuring the titratable acidity, I see titration of sample with 0.1 N NaOH to reach specific pH and reported by lactic acid. But the specifi pH I see is different in literiture,...
15 March 2018 8,141 3 View
My lab is interested in extracting RNA from sperm (small RNAs specifically), and I believe we have the correct kit (Zymo Quick-RNA Micro-prep), but we already have a lot of trouble with extracting...
06 August 2017 8,639 1 View
05 August 2017 5,723 5 View
I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...
28 February 2021 2,393 8 View
I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....
28 February 2021 7,967 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...
24 February 2021 4,618 4 View
24 February 2021 3,348 2 View
Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...
24 February 2021 9,578 4 View