The cells are genetically engineered and grown on PDL plates for a potency assay. My BCA is highly variable. I am wondering if triton disrupts the PDL coating, which then interferes in the BCA leading to the variability observed. Can this variability be a result of triton releasing lysine from the plate coating?
Non-ionic surfactant Triton X-100 can break down cell membranes to remove proteins and cause cell lysis. It breaks down hydrophobic connections to solubilize proteins and lipids. Bicicinchoninic acid (BCA) assay variability may be associated with Triton X-100's effect on PDL coating. Triton X-100 may interfere with the way proteins interact with one another, which could have an impact on how PDL binds to the plate. Try varying amounts, evaluate the stability of the PDL coating, run control tests, and think about other cell lysis techniques to learn more about this. To find the cause of the assay's variability, it is essential to troubleshoot and validate each step.
http://eprints.nottingham.ac.uk/56001
https://www.hindawi.com/journals/omcl/2020/7152173/
https://www.cell.com/biophysj/pdf/S0006-3495(21)02104-4.pd
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