I encountered the fluorescence enhancement in the DNA interaction process. Since the classical Stern-Volmer equation is used for the fluorescence quenching process, this equation cannot be used to calculate the fluorescence enhancement constant. How can I calculate the fluorescence enhancement constant? Are there any articles on this topic?
Thanks in advance.
I am not aware of a fluorescence enhancement constant, but I have been in situations where fluorescence enhancement had to be quantified in some way. One approach is to identify the concentration of the substance that produces a certain proportional increase, such as 2-fold or 3-fold. Another approach is to measure the full scope of concentration-dependent fluorescence enhancement, and then to calculate the concentration of the substance than produces 50% of the full change.
The second approach may be more complicated than the first, because each substance tested may enhance the fluorescence by a different amount, or it may not be possible to get to the maximum fluorescence due to insolubility of the substance or interference by the substance with the measurement.
Please check whether substance in question is itself fluorescent by repeating the titration without the DNA. If it is fluorescent, then you have to subtract the background fluorescence from the substance alone at each concentration.
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