I have to characterise fibroblasts and preadipocytes from fat tissue. MOst of the articles have used DMEM/ F12, while some have used DMEM. Is there any rational behind using specific media over the other?
After UVO treatment on PDMS, I got C=O stretch peak from ATR-FTIR. I used Sylgard184 (base:curing agent=100:4), and treated UVO 90 minutes on cured PDMS by UV ozone cleaner.
05 April 2024 1,902 0 View
I need to determine indentation and tissue stiffness. I already have paraffin embedded tissue, but not sure if that will give an accurate result or I should use a fresh tissue.
11 February 2024 2,114 0 View
I have to determine adipose tissue stiffness. My samples were preserved in NBF. Can I use those tissue sections? or should I use fresh tissue to identify tissue stiffness? Is there any protocol...
01 February 2024 7,882 0 View
Examples of informal publishing: Blogs Podcasts Letters/Email YouTube videos Examples of formal publishing: Peer-reviewed journal articles Books printed by scholarly publishers Conference...
10 October 2023 3,434 0 View
I am sorting preadipocytes from SVF and now need to differentiate them into mature adipocytes. Does a mixed cocktail of preadipocyte differentiation media works best of making a cocktail by...
07 October 2023 1,141 0 View
I have to differenitate stem cells into preadipocyte and further into mature adipocyte. We want to quantify ECM produced by adipocytes. Not sure if I should use Immunoflurence or there is any...
04 September 2023 6,458 0 View
Given the disproportionately high risk provided by blood-borne pathogens, regular monitoring of blood transfusions is required to avoid disease transmission. ELISA and Rapid diagnostic...
01 September 2023 7,864 1 View
As I know, there are 3 reference energy to find or compare band level when we need to band alignment data 1. fermi energy set to zero 2. Vacuum energy (from slab calculation) set to zero 3. Align...
03 August 2023 2,186 0 View
What's the hallmark of adipose tissue fibrosis? What techniques can be performed to identify and quantify adipose tissue fibrosis?
24 July 2023 2,704 0 View
I have to characterize fibroblast and myofibroblast in adipose tissue. Since these are connective tissue cells and ubiquitously present in all connective tissue therefore, isolation from a...
18 June 2023 1,307 1 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
I am using fibroblast growth factor, purchased in powder form. What suitable substance should be added to get it in a liquid form? Thanks
03 July 2024 9,865 2 View
Hello, everyone. I would like ask one question. I transfected fibroblasts with EF1A-myod-puro using lipofectamine 3000. Promoter for puro is CMV. After transfection 48 hours, I did puromycin...
23 June 2024 4,902 3 View
Greetings all, My group has been trying to reproduce 3t3-L1 cell (preadipocytes) differentiation. The typical protocol involves 3 steps: the first step and second step both last for 2 days, and...
18 June 2024 2,728 1 View
Bfgf was aliquoted in media and transported at 22 degee Celsius. How long it is stable
15 June 2024 3,010 2 View
Hi everyone, I am working with an adhesive fibroblast cell line in roller flasks (Cell Master Bottles). When culturing the cells with FBS that is heated with FBS (around 60 degrees C), the cells...
02 June 2024 7,409 1 View
I started a cell culture with a mix of suspended and adherent cells (presumably fibroblasts) on May 10th. After a week, I treated them for adherent cells only. On May 24th, most of the adherent...
02 June 2024 4,902 5 View
Recently I've been doing some indirect co-culture experiments, in a nutshell, treating cancer cells with CAF's conditioned medium. So how do you do the collection of conditioned media and the...
01 June 2024 9,483 2 View
I have a peculiar problem - in FBS or albumin containing media my cells (preadipocytes) are very easy to image (and therefore quantify using a combination of ilastik and CellProfiler). However,...
28 May 2024 3,780 0 View