Why should skim-milk be used for blocking buffer in western blots?

I have just performed my first western blot and none of my targeted proteins were detected. The only thing visible on the membrane was the prestained ladder. Someone suggested that my blocking...

21 August 2018 5,787 4 View

How to grid downscale CMIP5 climate data grid to 0.5 degree using Matlab ?

08 July 2018 2,235 2 View

Merging many to 1 in SPSS version 25?

I have 2 datasets one is the adult in the houshold and the other is the child(ren). I'm trying to merge the child data to the adult data however there may be more than 1 child to an adult (many...

10 May 2018 4,946 6 View

Why would LB-low salt medium be prefereable to normal LB medium?

I've come across a protocol that says to use low salt LB broth (10g/L tryptone, 5g/L NaCl, 5g/L yeast extract) for bacterial growth curves. Why would it be preferable to use a low salt medium...

18 February 2018 2,048 2 View

Why is the deletion of bcsQ using lambda red recombination unsuccessful?

I have been unsuccessful in knocking out bcsQ in Pectobacterium carotovorum ssp. brasiliense using a three-step PCR method. Plasmids used: pKD4 and pKD46 bcsQ is MinD homologue part of the...

17 July 2016 1,533 2 View

Has anyone seen this strange FSC-A versus FSC-H pattern?

I recently ran an experiment where I divided a mouse tumor into several wells of a 96-well plate. Each well was stained with a different panel of antibodies. In only one of the wells, I got the...

22 February 2016 4,653 6 View

Does anyone have suggestions about cell density, DIV and surface preparation for hippocampal neuron culturing for micro electrode array?

27 November 2014 4,362 2 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

How to determine the significance of "diagonal" contrasts in two-way ANOVA interactions on SPSS?

Hi all, I'm running a 2 x 2 between-subjects ANOVA with the setup in the attached image (kindly refer to JPG attachment). I understand that calculating simple effects involves determining...

09 February 2021 547 3 View

Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View

How to interpret multiple bell shaped curves in Kratky plot?

Hi, I'm trying to analyze a Kratky plot generated from my SAXS data. I am seeing multiple bell-shaped curves in my Kratky plot and I am not able to find any source to get relevant info on this...

20 January 2021 2,474 1 View

How to edit a post hoc Kruskal Wallis test in SPSS that has generated an unreadable table?

I have gathered field data about birds at hanging feeders. One question I am trying to answer is is the proportion of time spent being vigilant at the feeders the same in all species. I have run...

07 January 2021 8,445 4 View

How to increase the attachment of the HEK293t cells to the glass coverslips or cell dishes?

Hey guys, I always have problems with the attachment of HEK293t cells to glass coverslips while doing IFA. They are so easy to detach from the coverslips while washing. The coverslips are already...

30 December 2020 8,381 3 View

Variable with negative values in SPSS?

Hello, dear colleagues! I would ask you for a little help. Actually, I'm doing a multivariate linear regression analysis in the SPSS and one of the independent variables has negative values. In...

20 December 2020 535 2 View