Hello can any one please give the the BioXM software ?

i need this software in english version?

07 August 2016 4,204 2 View

Which restriction enzyme is best to use as i want to use pCAMBIA 3301 for expression analysis of a gene?

07 August 2016 9,672 3 View

Over-expression of a gene?

hello research gate. 1-how to study the over-expression of a gene ? 2-like in general, what things we should have to consider for studying the function of gene by over-expression? 3-which is a...

05 June 2016 5,363 1 View

How To find the family of a gene in soybean?

I have one gene and I want to know the family of that gene and is that possible to know the function of gene by using any of twee

05 June 2016 3,019 2 View

Using pcr product and do the pcr again ?

hello, can you please tell me if i use the pcr product as a template and do the pcr again so is there any mutation will occur in my 2nd pcr product but as i check the re pcr products by using the...

05 June 2016 1,004 5 View

Soybean mosaic virus use for transformation?

05 June 2016 1,820 1 View

How to design the primer from the CDS sequence?

how to design the primer from only the CDS sequence as i want to clone my gene into the expression vector?

05 June 2016 1,751 3 View

Concentration of PCR product?

Hello, can any body please tell me how to improve the concentration of PCR product?or how to increase the concentrations of gel purified/gel extracted product? my preference is to improve the...

05 June 2016 8,484 2 View

Is there any way to check the function of candidate gene by using any software?

i just want to know the predicated function of my gene can i do this by using any software?

05 June 2016 2,240 0 View

Can we use taq polymeraze to add poly A tails after finishing the pcr with HIgh fidelity DNA polymerase ,?

04 May 2016 3,284 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View