Trypsin is inactivated by the serum in your medium (more specifically, the proteinase inhibitors) you add after your trypsin treatment. Washing your cells with PBS after treatment with trypsin is therefor not needed.

Trypsin is inactivated by the serum in your medium (more specifically, the proteinase inhibitors) you add after your trypsin treatment. Washing your cells with PBS after treatment with trypsin is therefor not needed.

Hello

Traditionally the cells are washed first with PBS because most of the growth media for cell culture contains Fetal Bovine Serum (FBS). FBS contains protease inhibitors, such as α1-antitrypsin and α2-macroglobulin. These inhibitors stop the trypsin protease and act by inhibiting lysosomal peptidases that may be released, therefore no need to wash cells with PBS after treatment with trypsin

Good Luck 

hi, it is recommended to wash cells with PBS or serum free medium before adding trypsin to remove any residual serum that has an inhibiting effect on Trypsin as the former comments reported...then start trypsinization..... for your question: yes i think it's better to wash cell with PBS after using trypsin, we prefer to wash cells with the culture medium + FBS (complete medium), to assure the inhibition of Trypsin action... good luck

This is a very common cell culture question! The responders above have hit the nail on the head - in the majority of cases there is no need to wash with PBS/media as the FCS in your media will inactivate the trypsin. However, there is a small number of specific instances where it is necessary (mostly surrounding addition of low-serum or serum-free media); in these cases, add media to the trypsinised cell slurry and centriguge at a low speed to pellet the cells. Then, remove the supernatent, resuspend in a small volume of media, and add the required proportion of cells to a new flask

Some scientists perform this step after trypsinisation even when using media conatining FCS, but it really isn't necessary. As most people require visual evidence, you can get two flasks of your cell liine and perform a few passages using both techniques and see if you notice a difference in morphology, confluence etc

All the best

Thank you all for your responses to my inquiry, I really appreciate it.

All the best

Hello! I wash the cell with PBS before and after trypsinization just to clean the culture flask and to remove residual complete medium (before adding trypsin) and after, to remove cell debries that doesn't had detached by trypsin. Good luck!

Hello! Always, always  wash cells with PBS before and after trypsinization of cells, this is basic need to get rid of all the unwanted materials such as  serum, trypsin  and  other things from cells. 

Hi, trypsin is inactivated by the serum in your media. If your media if without serum or low serum you can also consider stopping trypsin action by adding the serum and then after centrifugation of your cells, resuspend them in your media.