Along with which virulence factors produced during that biofilm transition stage from the planktonic cells. Its good that it was found that cells experience shear stress and flow for formation of biofilms.
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Thank you Alireza Mordadi. I was very delighted to look through this paper. I eventaully looked at secondary metabolites time of production before biofilm production. It was something like 3-7 days of biofilm production in rat/ mouse modesl 2008. However the time of conversion from acute to chronic biofilm or from zero to chronic biofilm is uncertain.
Time of forming biofilm or quality of that depends on many factor, especially virulence genes. In my project toxin-antitoxin, I had many strains that isolated from patient but only some few one had ability to producing biofilm.