Along with which virulence factors produced during that biofilm transition stage from the planktonic cells. Its good that it was found that cells experience shear stress and flow for formation of biofilms.
Click Pubmed and in the square click on anything you like,you will find many papers
PubMed: "Pseudomonas aeruginosa"[title/abstract] and "biofilm"[title/abstract]
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https://www.ncbi.nlm.nih.gov/pubmed
Look at this
Critical review on biofilm methods
Article · Nov 2016 · Critical Reviews in Microbiology
Joana Azeredo · Nuno F Azevedo · Romain Briandet · Claus Sternberg
Thank you Alireza Mordadi. I was very delighted to look through this paper. I eventaully looked at secondary metabolites time of production before biofilm production. It was something like 3-7 days of biofilm production in rat/ mouse modesl 2008. However the time of conversion from acute to chronic biofilm or from zero to chronic biofilm is uncertain.
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