I am looking to immortalize skeletal and cardiac myoblasts without messing with p53. Skeletal myoblasts can be readily transformed with a combination of mTERT and CDK4 (I suspect that this will hold true for cardiac myoblasts too). I have plasmids encoding hTERT and hCDK4 on hand. My question is whether the human variants will work in a mouse system. Uniprot gives 94.7% sequence identity between human and murine CDK4, and 62.0% identity between TERTs.
Amos Bairoch
It even works with bovine and pig cells. As shown in:
http://web.expasy.org/cgi-bin/cellosaurus/search?input=24589663
where they have used hTERT and hCDK4.
Curiously I could not find an example in mouse, but there is no reason to think it would not work.
Good luck
Amos
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