We use Falcon 96-well assay plates for Saccharomyces cerevisiae growth cuves measuring OD600 under certain chemical conditions. Does anyone know if these plates can be cleaned, autoclaved, and reused? If so, how?
Thank you!
Polystyrene plastic will melt if autoclaved. These plates are not meant to be reused.
In our experience when you proceed to autoclave, and repeat an experiment, the results are not the same, if we make a triplicate of known samples, you can give only one or two reference values. In general terms, the reading of the plate is greater in all the wells. It depends on the use you want to give, but if you want precision and accuracy in a determination it is not advisable to autoclave them.
I don't think they are autoclavable. Also, it is not a very good idea to reuse consumables such as plates, tips, tubes etc... Even if you clean and autoclave them there still is a risk of contamination.
I think that 96 well plate is not equal to 96 glass tubes. "Penny wise and pound foolish". Further, 96 well-plate assay gives miserable false result due to violence against "The Lambert-Beer's Law".
I do not like Falcon 96-well assay and Nanodrop in biochemistry at all. HPLC-photometric method is unique to give the true result (please see Fig 10 in the file; SEC fucoidan determination).
HPLC-photometric method can reduce the solution volume to 0.05 mL, and use of high-cost drugs can be reduced. HPLC-photometric biotinidase assay by using biotinyl-6-aminoquinoline (BAQ) as substrate can safely be performed even after Sigma has stopped selling of BAQ (our unpublished experience; please see file, J Chrom B Rat BIN LIP Km).
Can you autoclave/reuse a Falcon 96-well assay plate?
NO, these plates cannot resist the autoclaving conditions....they cant be used again...
Regards
I honestly would not know to answer your question. It's a subject that doesn't compete in my area of study.
Regards.
Although I don't work with yeast, but I do use such plate quite often. I work with the protozoa Leishmania. Typically after use each time I extensively wash the wells with running DI water followed by extensive wash in 70% ethanol. Then leave the plate inside tissue culture hood over night with the UV on. This works perfectly fine for me.
96 well plates are not autoclavable and they will definitely melt. Falcon tubes might be autoclavable but is it really worth the time and the effort? a bag of 50 falcon tubes is like 4.50 pounds from some suppliers.
I have used these kind of plates for staining plant tissues transformed with GUS. Before throwing them away, we autoclaved them. They melt and deformed into a ball-shape.
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