Which method would detect smaller changes in protein synthesis in a cell?
Hello, I understand I need to use the equation c1v1=c2v2 to work out the concentration of Anti-puromycin 12D10 (0.5mg/ml) diluted by 1:1000 in PBS. However could somebody explain the equation to...
09 October 2017 6,311 3 View
I am investigating the puromycin-labelled protein in MSCs. I have taken the integrated density of the immunofluorescence results and Spot blot results. However, what scale do I put for the...
09 October 2017 2,241 1 View
09 October 2017 7,478 2 View
08 September 2017 3,854 3 View
My project is comparing Serum culturing for 30 and 60 minutes and serum free culturing for 30 and 60 minutes. Therefore I am comparing all 4 variable with 5 ratios for each treatment. What would...
08 September 2017 943 5 View
I need to compare data from the spot blot assay and immunofluorescence- (Integrated density from both) to determine the best method of analysing protein synthesis in serum (variable 1) and serum...
08 September 2017 933 2 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
