I am going to treat the myocardial infarction rats with conditioned medium for 8 weeks/56 days. It has often been administrated via tail over a short period of time. However, it seems that the tail protocol may not be possible for a long period of time.
What protocol do you recommend to administer that for 8 weeks?
Can the intraperitoneal injection and oral administration be right options?
Your advice and suggestions will be much appreciated and welcomed.
Hello Abdulbaset.
Make a cannulation cathether with 23~21G needle on tail vein for consecututive administrations from the root of the tail. After perfusion, add small amount of sodium citrate buffer to prevent from clogging. until next perfusion.
http://www.polymedicure.com/?wpccategories=i-v-cannula
Make another canulation site near to the first one if the first one clogs. You could reduce the number of administrations by increasing one dosage. However, IP injection needs verification of effective dosage and probably peptides released from the cell culture. Oral administration will completely destroy the peptides in the digestive tracts.
Best wishes.
Dear Abdul,
You can give IP injections but in that case you have to increase protein dose to around 10 times more than you planned to give by IV. I have done daily IP dosing before for 2 weeks for secretome and around 10 weeks for some drug. In both cases, results were as per expectations but increased the secretome doses to around 10 times for IP to counteract the reduced bioavialability.
Hope it helps!
Yes, the intra peritoneal good is an alternative. Also you can use a catheter or water bottle to feed test group of rats.
Dear all,
Thank you for taking the time to share all of that great information with me. Your support and advice have been extremely helpful.
Best wishes
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