Hi,
I am doing starvation assays with Leishmania parasites for which I need to culture parasites in PBS for about 6 hrs. Typically I find that without serum supplement these cells become very sticky and attached to the bottom of the culture flask.
I was wondering if anyone has ever applied the cellstripper solution (alternative of trypsin that is typically used to lift off adherent mammalian cells) on Leishmania or related organisim Trypanosoma?
Thanks in advance for your suggestions!
Sumit
Dear Sumit,
Attachment of Leishmania cells in absence of proteins is a recurrent problem to investigators.
I think the simplest measure to resolve your problem is strongly agitate your culture flask.
Promastigote forms are very resistant and you can employ this method to release most of the cells whitout kill them.
Sincerelly yours,
Nelder.
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