Sequencing of human genome at the center of interest in the biomedical field over the past several decades and is now leading toward an era of personalized medicine. During this time, DNA sequencing methods have evolved from the labor intensive slab gel electrophoresis, through automated multicapillary electrophoresis systems.
Reports available covers separation mechanisms based on free - zone capillary electrophoresis , Ogston sieving, and reptation. Two prevalent gel matrices ffor gel - facilitating sieving, which are linear polaryylamide and polydimethyacrylamide, are compared in terms of performance, cost, viscosity and passivation of electroosmotic flow. . The role of capillary electrophoresis in the discovery, design, and characterization of DNA aptameters for molecular recognition is discussed . There are many expanding and emerging techniques in the field. For more information consult https://www.ncbi.nlm.nih.gov also check Anal Bioanal Chem. 2015: 407: 6923 - 6938
Hi Mushtak,
it's simple but very reliable for small studies as we known. there is two types of electrophoresis, agarose gel electrophoresis, and the second used by old AB sequencers, polyacrylamide gel. for what you asked, the gel (POPn) is included in capillaries by trough your samples will migrate. since your samples (bands or sequences) are fluorescent (4 dyes are used), they will be detected by the CCD camera at end of electrophoresis and interpretation of sizes will be done.
fred
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