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Questions related to Molecular Biological Techniques
I measure plasma MDA & GSH level in healthy men, but I can't identify normal level, decreased level or increased level of MDA/GSH.
26 November 2016 6,609 1 View
i have 3 groups, negative control, injected normal cells, injected mutation cells. when i got the counts/sec, i found the control group is also high, do i need to use cells group to divide NC...
24 November 2016 1,624 1 View
I used culture of PBMC supernatant to detect drug-restricted cytotoxic protein of granzyme B. The samples were from PBMC of (1) patients of drug remission, (2) patients of drug tolerant, and (3)...
23 November 2016 3,133 0 View
Is there anyone who ever used dual-crosslinking method in ChIP experiment? I want to ask if there are any subsequent effect on the Chromatin fragmentation step, and because increase the spacer...
22 November 2016 4,909 3 View
Hi, can anybody send me an optimized protocol for short fragment of sscp pcr (117bp) using sliver staining?
22 November 2016 7,673 3 View
We are trying to isolate CD63+ exosomes in order to characterize them by electronic microscopy, Nanosight and ultimately by proteomics/transcriptomics. To isolate them we use the “Total Exosome...
21 November 2016 1,249 16 View
There are so many microbial diversity investigations using high-throughput sequencing on variety of natural environments, e.g. land soil, fresh and salt water, sediment etc. Some of them sampling...
21 November 2016 5,057 4 View
I cut pcmv6-AC-ha-his(origene) with hind111 and xho1, the same as my insert whose size is about 1k. Then I ligate them which labeled as "target" ,and negative control is the ligation system which...
15 November 2016 7,242 3 View
I'm trying to isolate RNA from suspended adipocyte now. The problem is that after centrifuging, the cells were still floating at the bottom, so when I remove all the media before adding RLT...
14 November 2016 2,340 4 View
I did DNA extraction of cyanobacteria. Up until the very end it seemed to be working fine. The Chloroform/isoamly alcohol (24:1) steps all formed a separate layer when introduced and seemed to be...
09 November 2016 7,352 6 View
I want to detect gene expression by multiplex PCR. The multiplex PCR reaction works fine on genomic DNA, but on a cDNA template the same oligo mix seems not to work as nicely, although specific...
07 November 2016 8,802 5 View
I just did my first RNA extraction from tissue using a trizol-like reagent called "Tranzol". I must have eluted it to 70ul final volume but I'm having poor yields of approximately 150ng/ul, low...
31 October 2016 6,698 5 View
Recently, i'm doing a sonication to bacterial pellet using PBS 10x buffer. I'm being confused in adding the buffer to the pellet for sonication. I'm setting the amplitude for 20-30%. Is there any...
28 October 2016 9,118 2 View
How to make Tris Succinate buffer (0.05M, pH8.2)?
26 October 2016 5,293 3 View
I tred to dissolve vinclozolin for cell culture use. I dissolved it in DMSO. There was no problem in this step. For further dilution, I was used full medium (DMEMF12+FBS), and water, seperatly....
25 October 2016 3,892 1 View
I want to constructed Crispr Cas system in archeae and I need to know which promoter and terminater I should use to express RNAguide. Indeed, if I understand well, promoter should be one for tRNA...
18 October 2016 1,471 3 View
Hi. How can I remove endotoxin from bacteriophage lysate with a simple and high-efficiency method?I will appreciate to help me.
18 October 2016 4,850 1 View
Is amino benzoxymethyl paper the best choice?
16 October 2016 2,153 3 View
We want to do the experiments in hypoxia and came across rather huge usage of nitrogen gas for it...
16 October 2016 2,561 1 View
would anyone have information to how the PBMC pellets can be stored?after Ficoll and washing with PBS or HBSS can the PBMC pellet be stored frozen in -80deg? or does it have to be resuspended in...
12 October 2016 4,832 2 View
Read 7 answers by scientists to the question asked by Ramya Murugesan on Oct 6, 2016
06 October 2016 9,947 6 View
I am working on Ub-MDM2/MDM 2 assay kit. Any experience shared will be appreciated.
03 October 2016 4,136 1 View
Our lab is using the Gene jet miniprep kit for plasmid Isolation. Somehow, the resuspension solution or alkaline lysis I buffer or Buffer p1 is going to empty soon even though we still have a lot...
18 September 2016 7,710 3 View
Hi, I'm looking for primers specific to Enterobacter cloacae complex or the entire Enterobacter species. The idea is to distinguish its members from other enterics by PCR. Does anyone know a...
13 September 2016 275 5 View