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Questions related to Molecular Biological Techniques
In most of the research papers reported till now on scaffold have shown cell bio-compatibility check using live/dead assay in frequent intervals till 21 days. Even when we are going for...
17 February 2023 9,555 0 View
Dear all, I would like to know how you prepare viscous cosmetic products such as serums, creams etc. for in vitro assays such as cell viability, ELISA etc.? I want to know how you adjust the...
17 December 2022 1,102 7 View
Include some comparative analysis of toxicity reduction according to different microbial synthesis routes.
10 December 2022 4,222 1 View
CdSe QDs green synthesis has been done by fungus or bacteria, but not synthesized by plant extract.
07 October 2022 712 0 View
I would like to measure formate production in a hyperthermophilic archaean but the formate assay kit I wanted to use has a shorter shorter shelf life than the duration it will take me to collect...
12 August 2022 6,873 0 View
my study has 3 mean and SD of 3 different areas on the body in the control group and the same for the experimental group. i want to sum the 3 calculations of each group, so i can calculate the SMD...
31 May 2022 7,851 3 View
How to calculate normative valves from Mean and standard deviation.
04 April 2022 5,313 3 View
Hello. I have an n-decane which is not certified reference material. In the bottle appear -30°C as the melting point. To calibrate DSC, should -30°C be compared with onset, peak temperature, or...
25 March 2022 3,685 1 View
I recently cultured a Microcystis aeruginosa strain, however it was contaminated by bacteria. I have tried to use solid medium to purify it. I used 0.4% agarose as the gel, and sterilized the...
24 March 2022 1,722 7 View
Induction at gestation day 7 led to abortion and loss of pregnant mice. My suspicion is that my route of exposure intraperitoneal at 5mg/Kg was too high and this has been used previously by other...
18 February 2022 9,594 1 View
I have to use the compounds for several assays, thus need to preserve them for altleast 6 months
09 September 2021 5,277 3 View
I have rat adipose-derived mesenchymal stem cells and the cells that are treated with maintenance media produced more alkaline phosphatase and stained more with Alizarin red than my ASCs treated...
14 May 2021 4,562 4 View
I bought two kinds of nanoscale black phosphorus(BP)——BP Quantum Dots(1~10nm) and BP Sheets(100nm~1um). I wanted to see how cytotoxic they were, but when I added them to the medium there was very...
09 March 2021 4,646 2 View
Media preparation for In vitro selection against salt stress
27 December 2020 4,149 2 View
Hi everybody, I am working with a RAW 264.7 cell line to test the mRNA expression of the proinflammatory cytokines. Right now, I'm having some problems, hoping to get some useful advice. RAW 264.7...
13 October 2020 6,555 7 View
Heavy metals mostly have gradually decreased conc. with gradual rise of Soil PH and vice versa except in case of lead hence the mobility can also be controlled in such way to get rid of toxicity...
14 August 2020 3,220 4 View
1. Determination of amino acids? 2. Determination of soluble Sugars and Protein? 4. Determination of antioxidant enzymes (CAT, POD and SOD) and synthases (PPO and PAL)? 5. Determination of MDA...
10 August 2020 7,841 7 View
Dear members, As we all are aware, BKC is very common preservative used in various opthalmic dosage foms. I want to know, some basic information with respect to BKC. As it has 4 homologs, and...
11 June 2020 5,225 0 View
I followed a protocol that I saw in many papers to promote liver injury by ethanol challenge in C57bl/6 mice but my results are completely different. The levels of ALT agree with the papers but...
13 February 2020 4,560 0 View
What's the difference between protein denaturation and degradation? During drying or preservation methods, what effects may it have on the fish protein?
09 February 2020 4,172 3 View
can somebody share his academic goals and career development after Fellowship?
03 January 2020 1,638 3 View
Using dobutamine in physiological measurements at 1µM it is evident that the compound becomes inactive within hours in Tyrodes at 35 degrees. What should I do to preserve dobutamine?
06 December 2019 4,416 0 View
I found the protocol and formula of Sedgewick rafter cell but I am confused in substituting the values into the formula so, I need your help in understanding how to use the formula while analysing...
26 October 2019 8,138 6 View
I want to immunize mice with Alternaria extract and OVA to induce type 2 immune response, activating Th2 cells. And I want to simply the route of immunization, immunizing mice by...
10 October 2019 9,356 1 View