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Questions related to Molecular Biological Techniques
Hello everyone, I was wondering how long before exosome/EV isolation should I withdraw human platelet lysate (serum) from cell culture media (considering mesenchymal stem cells)? I know that the...
06 November 2023 6,061 2 View
I am using 12% gel. Tissue was lysated using RIPA buffer and doing semidry transfer.
20 October 2023 6,138 2 View
Hi everyone, I am working on microalgae I am finding difficulty in maintaining the cultures. After 20 days microalgal cultures are turning brown. I used antibiotics, garlic extracts but not...
11 October 2023 5,178 5 View
I am looking for suitable protocols.
28 September 2023 2,306 1 View
How heat is transferred inside the Earth through convection currents and type of energy transfer is involved in the ground heating the air above?
24 September 2023 8,190 3 View
Exploring the feasibility and effectiveness of using digital platforms to disseminate agricultural knowledge and resources to remote areas in Bangladesh.
24 September 2023 3,775 1 View
Hi! I will be performing transformation in E.coli Stbl3 strain followed by plasmid extraction. However, the Stbl3 strain is EndA+ strain of E. coli . Can someone please guide me with some...
13 September 2023 8,664 3 View
I am trying to choose a strain for expression of a plasmid. Which one would be better?
07 September 2023 6,728 1 View
After overnight (12-16 hrs) incubation of E. coli in LB, when I attempted to extract DNA using Kit, it yielded a low concentration of DNA (10-20 ng/µL). Can anyone help me out to improve its...
28 August 2023 6,969 5 View
Hi everyone, I am doing auditory brainstem response measurements using powerlab AD instruments, but I am not getting the responses for lowest dB level as they are too weak to be measured by...
14 August 2023 3,383 0 View
I am in isolation process of zymomonas mobilis bacteria. So i need to know that there is any media for selectively isolate the zymomonas sps.
10 June 2023 3,978 0 View
Feel free to help me figuring out.
29 May 2023 4,840 0 View
In most of the research papers reported till now on scaffold have shown cell bio-compatibility check using live/dead assay in frequent intervals till 21 days. Even when we are going for...
17 February 2023 9,685 0 View
I have extracted dragon fruit flesh by using methanol to get the betalain pigment. Then I want to dissolve this pigment into poly lactic acid solution (PLA dissolve in chloroform). But the pigment...
20 January 2023 9,904 1 View
Dear all, I would like to know how you prepare viscous cosmetic products such as serums, creams etc. for in vitro assays such as cell viability, ELISA etc.? I want to know how you adjust the...
17 December 2022 1,238 7 View
Include some comparative analysis of toxicity reduction according to different microbial synthesis routes.
10 December 2022 4,371 1 View
CdSe QDs green synthesis has been done by fungus or bacteria, but not synthesized by plant extract.
07 October 2022 835 0 View
I would like to measure formate production in a hyperthermophilic archaean but the formate assay kit I wanted to use has a shorter shorter shelf life than the duration it will take me to collect...
12 August 2022 6,981 0 View
my study has 3 mean and SD of 3 different areas on the body in the control group and the same for the experimental group. i want to sum the 3 calculations of each group, so i can calculate the SMD...
31 May 2022 7,970 3 View
How to calculate normative valves from Mean and standard deviation.
04 April 2022 5,440 3 View
Hello. I have an n-decane which is not certified reference material. In the bottle appear -30°C as the melting point. To calibrate DSC, should -30°C be compared with onset, peak temperature, or...
25 March 2022 3,809 1 View
I recently cultured a Microcystis aeruginosa strain, however it was contaminated by bacteria. I have tried to use solid medium to purify it. I used 0.4% agarose as the gel, and sterilized the...
24 March 2022 1,860 7 View
Induction at gestation day 7 led to abortion and loss of pregnant mice. My suspicion is that my route of exposure intraperitoneal at 5mg/Kg was too high and this has been used previously by other...
18 February 2022 9,731 1 View
I have to use the compounds for several assays, thus need to preserve them for altleast 6 months
09 September 2021 5,443 3 View