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Questions related to Molecular Biological Techniques
Planning to express two sets of genes separated by 17 nucleotides. Have upstrem sequence but really dont know whether the two genes have same promoter or different ? I expect that they will...
08 August 2016 2,381 2 View
Dear All Hi, I am writing to ask your opinion about chance of success designed real time Taqman probe as: 5'- TAC GCC GTA YCT AGC TWY YGC CTG GAC CGT C -3’ as you see in about middle of probe...
08 August 2016 9,544 2 View
I have Medroxyprogesterone acetate in powder form which I want to dissolve it in DMSO but, I am not sure what concentration of DMSO I require.
08 August 2016 6,879 3 View
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07 August 2016 5,896 5 View
I used a TOPO TA kit which contained a linearized vector of 3.9kb to ligate my DNA of about 3.8kb. After transformation and plating on LB agar containing X-GAL I got quite a number of white...
07 August 2016 9,897 3 View
Specifically 25 uM Sigma I2764 ML-7 in DMEM low at 37 degrees C.
04 August 2016 6,248 1 View
in-fusion cloning is In-Fusion® HD Cloning Kit by Clontech® Laboratories
04 August 2016 454 2 View
i am using stripping buffer for 1hr to remove the previous bands but still the previous band are visible. is it possible to completely remove the previous bands with stripping buffer ? .
04 August 2016 2,485 3 View
I need isolate the CDV in cell culture.
03 August 2016 2,411 3 View
I prepared a 50 mM phosphate buffer in H2O and pH'ed it to 7. I lyophilized it and dissolve it in D2O? What is the pD? And what would be the pT (I would like a tritium water solution, ultimately).
03 August 2016 1,297 3 View
Is there anything I can do to reduce the amount of fuzzy and extra bands that appear behind my DGGE? Its making it difficult to differntiate between the bands I need. The standards are located at...
03 August 2016 8,033 4 View
I will be running my first luciferase assay and the protocol I am using does not specify what filters or filter settings I should use. The plate will be read on a lumometer from Biotek, Synergy...
02 August 2016 2,962 1 View
I expressed a protein in SUMO vector that express SUMO fusion protein, so, I wanna do an enzymatic essay after the protein purification without cleavage the SUMO protein. Has someone experience in...
02 August 2016 7,366 5 View
I made the siRNA and Lipofectamine2000 mix and incubated 20 minutes @ room temperature. The problem now is the cells are not healthy to start the transfer. Anybody please let me know how long i...
02 August 2016 4,577 3 View
I've been trying to clone a gene from H. influenza. Every step (PCR, digestion, ligation) seemed to work based on agarose gel images. Before I sent the DNA for sequencing, I digested the plasmids...
02 August 2016 10,009 5 View
Why C-terminal tagging of GFP did not result in the higher level of the protein expression compare to N-terminal tagging?
02 August 2016 1,577 3 View
Hi all, I am currently ligating 1.3 kb gene to pET29 vector, however, i cant get any bands after colony PCR using T7/T7t primers. I gotten a clear one band in RE digestion for clean cut vector and...
01 August 2016 9,808 4 View
Are all the bands plasmids itself?or is it degraded plasmid?
31 July 2016 7,164 11 View
Total Protein: 6His+MBP+TEV+tagret Protein: pI=6.49, MW=165.315 kDa,Cleaved Protein: target Protein: pI=8.42, MW=121.625 kDadialysis buffer: 20 mM HEPES pH 7.5, 50 mM NaCl, 10% glycerol. (similar...
29 July 2016 1,793 7 View
Does anyone know the best way to visualize ssRNA in gel electrophoresis?
29 July 2016 5,607 4 View
please also suggest articles regarding the same.
29 July 2016 1,205 1 View
Recently we have a condensate in our incubator. I'm worried how will it affect the cells, since it will definitely change the humidity levels in the chamber. Did anyone have this problem? I don't...
29 July 2016 8,565 4 View
Is cell lysis protocol same for all proteins?.
29 July 2016 1,066 3 View
I am new to PCR and digging into learning the roles of the different components in a PCR based on the FastStart HF PCR System I am using from Roche. If EDTA is a chelating agent, and we need the...
28 July 2016 737 4 View