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Questions related to Molecular Biological Techniques
Hi! I'm trying to perform a ChIP assay on SH-SY5Y cell line. Can anyone suggest me which duo of antibodies and primers to use for a positive control of ChIP? Also has anybody an experience of a...
20 January 2017 7,522 2 View
I have three types of samples including liquid samples (water, milk), solid samples (feed, feces) and aerosol samples. After quantifying by qPCR, I have the copies number of each sample. However,...
19 January 2017 10,050 8 View
Hello (sorry for long text) I've tried the isolation in three different ways, in the second trial I changed some steps of first trial which are decribed by “ / ” after related step, in the third...
19 January 2017 6,711 8 View
Hi, How i can remove polysaccharides and polyphenols during the DNA extraction from Terminalia arjuna, i have tried various methods but unable to find DNA in purified form? I have tried many...
18 January 2017 7,925 2 View
Hi all, This seems to be a fundamental thing but I have this doubt. I have biological replicates of a timecourse experiment with a particular treatment. I wanted to know if I can combine both to...
17 January 2017 4,855 3 View
RNA profile for all my samples shows strange big peak at 5S position. Should I use these RNA samples for downstream experiments? Does anyone know if this is common with Staphylococcus...
17 January 2017 1,551 5 View
I need to make a 1% casein agar for a protease assay, but I'm finding it very difficult to get the casein to dissolve. The media needs to be at ~pH5.8. It is used in several published papers, but...
16 January 2017 7,762 1 View
Hi everyone! do you have any piece of advice about RNA extraction from microvescicles? I usually recover MVs from human monocytes supernatant. I tried different protocols, like trizol and columns...
16 January 2017 8,098 4 View
I am trying to prepare agar plates from the CHROMagar powder and the instructions say to mix 33g in 1L of water and bring to the boil at 100 degrees before autoclaving. This is difficult for me as...
16 January 2017 3,291 3 View
Hello, the issue I am facing is I would like to put a his tag on the N-terminal of my GPCR, but it has a signal peptide for the first 20 or so amino acids and I am unsure of how to get around this...
16 January 2017 6,469 3 View
I plan to use Lipofectamin 2000 to transfect human macrophage in vitro. Macrophage is not a easy target for tranfection and there are not many papers on tihs topic. Does someone has some...
13 January 2017 9,868 4 View
We are currently using the digestion solution below to digest cells and matrix grown within 2% agarose gels however large pieces of agarose still remain. Any suggestions on how to trouble-shoot or...
13 January 2017 1,176 3 View
Hello everyone! I'm starting a project involving phage display and the protocols largely sound like I can do my panning on the bench top, instead of in a biosafety cabinet. Do I need to pan...
12 January 2017 2,687 1 View
I want to see the community structure of the soil amended with biochar, however, I've got difficulty in extracting high DNA yield. My samples did not pass the quality control for next generation...
12 January 2017 6,222 18 View
Hi, I had set up PCR with 50ng of FFPE and i was able see band at expected size. when i tried to repeat the same PCR with 50ng each in 4 vials i:e to bulk up the product. i did not get any band...
12 January 2017 3,082 1 View
In general from a confluent flask first I trypsinize and pellet down the cells, re suspend in 2 ml of media.Then I take 100ul of cell suspension and add 4 volume of trypan blue.After 5 mins, the...
11 January 2017 6,880 4 View
Hello Everyone, I am trying to develop a protein expression and purification system. Hence, can anyone tell me about "Intein Tags" or related papers, articles or suggestion? Please Help Thanks
09 January 2017 3,188 3 View
Hi all! While doing my DNA isolation I encountered a new problem. After the addition of chloroform and during centrifugation, the ependorff and 50mL centrifuge tubes melted. Can anyone provide me...
09 January 2017 2,787 12 View
Details: IMAGE ATTACHED Load 50ug protein in 20ul (All samples denatured for 10mins at 75% Precast 12% thermo gels Run 90volts 15mins (stack) 150volts for remainder of gel. Also run on...
09 January 2017 4,095 9 View
I am going to express Human CD36 on a mammalian cells, so I wonder if there's a company that I could buy the construct in a plasmid, and if they also provide positive and negative controls. Regards
04 January 2017 8,134 2 View
I am looking for rough recommended concentrations but I have only found in vitro treatment concentrations.
02 December 2016 6,441 1 View
I am currently trying to prepare a library for illumine deep sequening using the yeast Candida glabrata. Ive been having difficulty generating a high quality library. My collaborators believe that...
28 November 2016 1,062 1 View
I measure plasma MDA & GSH level in healthy men, but I can't identify normal level, decreased level or increased level of MDA/GSH.
26 November 2016 6,516 1 View
i have 3 groups, negative control, injected normal cells, injected mutation cells. when i got the counts/sec, i found the control group is also high, do i need to use cells group to divide NC...
24 November 2016 1,580 1 View