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Questions related to Molecular Biological Techniques
I have rat adipose-derived mesenchymal stem cells and the cells that are treated with maintenance media produced more alkaline phosphatase and stained more with Alizarin red than my ASCs treated...
14 May 2021 4,609 4 View
I have a question about a DSC thermogram, obtained from an analysis conducted with heating and cooling at 10°C/min, after deleting the thermal history. I'm sure that the main component is...
24 March 2021 4,302 4 View
I bought two kinds of nanoscale black phosphorus(BP)——BP Quantum Dots(1~10nm) and BP Sheets(100nm~1um). I wanted to see how cytotoxic they were, but when I added them to the medium there was very...
09 March 2021 4,700 2 View
I'm a student in M2 i hava a thesis to prepare about bdelloid rotifers. Please do you have any documents about the key of identification of the genera of bdelloid rotifers Thank you so much Good...
26 February 2021 7,478 0 View
Media preparation for In vitro selection against salt stress
27 December 2020 4,194 2 View
Can we deal with inactivated viruses in a normal lab with a biosafety cabinet (level1)? Is there any chance to get infected because of any accidental exposure to inactivated viruses? Any idea...
13 December 2020 8,328 3 View
Hi everybody, I am working with a RAW 264.7 cell line to test the mRNA expression of the proinflammatory cytokines. Right now, I'm having some problems, hoping to get some useful advice. RAW 264.7...
13 October 2020 6,601 7 View
Hi would like to know when PBMCs cells where harvested from the whole blood, and when treated with supernatant of probiotic strain fermented cereal solution for a period of 24 hrs ended up with...
03 September 2020 7,015 3 View
My lab needs to grow several strictly anaerobic bacteria that are of Biosafety Level 2. We need to use the Hungate technique (example:...
17 August 2020 7,477 1 View
Heavy metals mostly have gradually decreased conc. with gradual rise of Soil PH and vice versa except in case of lead hence the mobility can also be controlled in such way to get rid of toxicity...
14 August 2020 3,264 4 View
1. Determination of amino acids? 2. Determination of soluble Sugars and Protein? 4. Determination of antioxidant enzymes (CAT, POD and SOD) and synthases (PPO and PAL)? 5. Determination of MDA...
10 August 2020 7,896 7 View
I just want to find critical wavelength, in which 90 percent of the UV absorbance occurs. I know how to integrate my data, but don't know how to find the point where my 90 percent of data...
23 June 2020 4,513 0 View
Dear members, As we all are aware, BKC is very common preservative used in various opthalmic dosage foms. I want to know, some basic information with respect to BKC. As it has 4 homologs, and...
11 June 2020 5,265 0 View
Quantum dots (QDs) is growing interest for biomedical applications. At the same time, many scientists are convinced that QDs will never be used for treating patients because of their potential...
26 March 2020 6,407 7 View
I followed a protocol that I saw in many papers to promote liver injury by ethanol challenge in C57bl/6 mice but my results are completely different. The levels of ALT agree with the papers but...
13 February 2020 4,608 0 View
What's the difference between protein denaturation and degradation? During drying or preservation methods, what effects may it have on the fish protein?
09 February 2020 4,214 3 View
can somebody share his academic goals and career development after Fellowship?
03 January 2020 1,704 3 View
Using dobutamine in physiological measurements at 1µM it is evident that the compound becomes inactive within hours in Tyrodes at 35 degrees. What should I do to preserve dobutamine?
06 December 2019 4,455 0 View
I found the protocol and formula of Sedgewick rafter cell but I am confused in substituting the values into the formula so, I need your help in understanding how to use the formula while analysing...
26 October 2019 8,195 6 View
I want to immunize mice with Alternaria extract and OVA to induce type 2 immune response, activating Th2 cells. And I want to simply the route of immunization, immunizing mice by...
10 October 2019 9,392 1 View
I want to immunize mice with mixture of Alternaria extract and OVA to specifically induce type 2 immune response, activating Th2 cells. More importantly, I want to simplify the route of...
10 October 2019 3,206 2 View
Hello everyone I have a general question regarding generation of the Venn diagram. I would like to create a Venn diagram to see the overlapping genes that are commonly expressed between seven...
07 August 2019 2,877 6 View
I would like to study the microbial biodiversity of a hypersaline water by PCR-DGGE followed by sequencing. How to choose the suitable primers for both archaea an bacteria.
07 August 2019 3,563 4 View
How I evaluate this equation and what is the blank or control?
19 July 2019 9,228 3 View