Molecular Biological Techniques

Contact experts in Molecular Biological Techniques to get answers

2,092 views 3,284 posts

Questions related to Molecular Biological Techniques

Whether Intraperitoneal injection mice with Alternaria extract and OVA can induce type 2 immune respnse?

I want to immunize mice with mixture of Alternaria extract and OVA to specifically induce type 2 immune response, activating Th2 cells. More importantly, I want to simplify the route of...

10 October 2019 3,158 2 View

How to creat Venn diagram from RNA seq data contains 7-lists?

Hello everyone I have a general question regarding generation of the Venn diagram. I would like to create a Venn diagram to see the overlapping genes that are commonly expressed between seven...

07 August 2019 2,818 6 View

How to choose the suitable primers for archaea and bacteria in hypersaline water?

I would like to study the microbial biodiversity of a hypersaline water by PCR-DGGE followed by sequencing. How to choose the suitable primers for both archaea an bacteria.

07 August 2019 3,503 4 View

How can i calculate SOD, MDA, GSH, Catalase, GPx for EX vivo?

How I evaluate this equation and what is the blank or control?

19 July 2019 9,153 3 View

What are the chances that sulfamethoxazole and trimethoprim in water is degraded after long time preservation?

Hi all, I preserved my water samples (obtained from plant rhizosphere) at 4 °C two years ago that were contaminated with sulfamethoxazole and trimethoprim. I am wondering if I can still use them...

17 May 2019 8,566 1 View

Protocol for storage o washed erythrocytes for enzymatic antioxidant analyses?

Hi all, I've been reading and looking for some protocols of storage of erythrocytes for enzymatic antioxidant (SOD,CAT, GPx) measurement, however, I found plenty of information that bring me...

07 May 2019 5,752 5 View

How do I resolve this issue with C. albicans LiAc transformation?

Hi everyone, A while back a lithium acetate transformation of a triple knock out mutant Candida albicans strain was performed yielding several transformants. The transformants, however,...

07 April 2019 4,386 5 View

Can a free ion generate a AC-susceptibility signal at frequency about 5000 Hz?

Can a free ion (Fe3+) generate a AC-susceptibility signal at frequency about 5000 Hz?

19 March 2019 7,360 0 View

Is there any free NASGRO training resources available?

Crack growth calculation

02 February 2019 3,108 0 View

Can some one add paper about Genes regarding to SOD, POD, CAT, APX, MDA, and melatonin biosynthesis gene under heavy metal stress in cotton or other?

Dear researchers, I want to find some genes related to SOD, POD, CAT, APX, MDA, and melatonin biosynthesis gene under heavy metal stress in cotton or other crops? Kindly upload the related...

19 October 2018 5,668 2 View

Asking reasons for the death of intraperitoneal-OVA-immunized balb/c female mice ?

In my protocol, balb/c female mices were divided into two groups : 1. PBS group 2. OVA group which were immunized with PBS or OVA antigen respectively by introperitoneal injection on day 15, 25,...

13 September 2018 1,917 0 View

Prices of LC-MS systems?

We have the occasion to bid for some LC-MS systems, but I have no idea of list rpice of the systems.One is a Sciex Triple Quad 6500 with an Agilent MS-40+ vacuum pumps, a shimadzu LC system...

23 July 2018 6,087 6 View

How to prepare standard curve of MDA using TMP for TBARS assay?

I want to measure the TBARS values of edible oils after oxidation. Can anybody help me to prepare MDA standard curve using TMP. I am using the 1 butanol as solvent. Further I want to know the...

29 March 2018 807 0 View

Looking for an online tool to make sequence-secondary structure diagrams for unpublished protein structures?

Dear all, I am looking for an online tool (or software) that can generate sequence-secondary structure diagrams of unpublished protein structures? I have found plenty of tools that either predict...

21 March 2018 1,506 4 View

Help for the calculation of phytoplankton density?

I am making corrections to a database of observations of phytoplankton samples. the point is that he needs to convert the observations into density (cel / ml). I found a formula that mentions. N =...

07 March 2018 5,665 1 View

How to determine SOD, CAT, GSH, MDA, LOOH, SH-groups in serum of the cirrhotic patients?

To determine SOD, CAT, GSH, MDA, LOOH, SH-groups of proteins, 4-HNE in case of liver cirrhosis - will it be the best to use serum? How many time this serum can be stored in the refrigerator...

02 March 2018 2,531 10 View

How to assign Taxonomic Classification for OTU tables?

I am new to metagenomics. I downloaded a 16s rRNA data from sra and generated OTU table using USEARCH. How to assign Taxonomic classification to OTU table. I tried with the following...

18 February 2018 7,753 2 View

Immunohitochemistry antibodies for osteoblast?

hi, I would like to know which antibodies serve for immunohistochemical identification of osteogenic markers in differentiated osteoblasts of mouse adipose-derived mesenchymal stem cells or where...

12 February 2018 5,229 5 View

What is the meaning of big black dots (which seems to be DAPI negative) in a DAPI stained nucleus of a C2C12 cell?

I am looking for nuclear localisation and function of a protein that is FITC tagged in the pictures attached.

11 February 2018 9,356 2 View

What is the effect of Cadmium Chloride on Pancreas of albino rat ?

Cadmium, a toxic heavy metal is an effective shortand lonCadmium a toxic heavy metal with short and long term effects particularly showing accumulation in organs as kidney, liver brain and testis....

21 January 2018 1,002 3 View

Anyone having experience of working with ATTC 5736 and T24 bladder cell lines?

I am having problem obtaining the desired confluency. They are not growing out well even after 24-72 h incubation. Confluency remains less than 60% Note: - Media is RMPI 1640 (As recommended by...

16 January 2018 1,046 4 View

Oxidative stres cases?

How can we interpret these data: 1st case a significant decrease in MDA, H2o2 and a significant increase in SOD, while GSH and NO are not changed 2nd case A significant decrease in No, H2o2o, and...

27 December 2017 6,474 2 View

What happen within an algal cell if the SOD, CAT and MDA activity decreases upon exposure to heavy metals?

Mechanism of action after exposure of heavy metals

20 November 2017 3,376 9 View

How can I achieve spore germination of Blumeria graminis f.sp. hordei within a simple test to count germ-tubes after e.g. 24 h?

It should be possible to induce germination also on slides or agar plates. I have already tried different methods: aqueous solution, film of water on slide, nutrient solution with dextrose,...

02 November 2017 4,876 2 View

Badges
Science topic