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Questions related to Molecular Biological Techniques
Read 2 answers by scientists to the question asked by Vinu S Siva on May 1, 2016
01 May 2016 1,368 2 View
What experiments can I design to provide insight into the position of my gene of interest (Sif2) in the process/pathways (eg. upstream or downstream)?
01 May 2016 2,674 2 View
several researcher used different technique.
28 April 2016 5,888 2 View
We have tried various modifications to our standard Western Blotting procedures now trying to detect CHOP (GADD153) in our human cell lysates after e.g. stimulation with 1 µg/l tunicamycine, but...
27 April 2016 2,914 4 View
Hello everyone, we just bought EZ-Vision Three DNA Dye as Loading Buffer, 6x for visualizaing our gels. Even though we are applying 1part of EZ-vision to 5parts of DNA as the protocol explains,...
27 April 2016 9,066 1 View
I would like to measure ROS levels or other oxidative stress marker in culture media from human stem cells. Does anyone have a protocol? Do I have to use fresh media or can I use also frozen...
27 April 2016 9,300 7 View
is there somebody have experience in FRET analysis, i recently use Leica SP8 confocal doing the FRET to detect the protein protein interaction, by using a positive Ctrl, Venus-Cerulean, we keep...
27 April 2016 4,975 7 View
What is the usual recommended procedure for maintenance of the PFGE electrophoresis cell during long breaks (not in use for 3 months): 1. Clear all the buffer/water from tubings, OR 2. Flush once...
27 April 2016 3,000 7 View
I've been working on nucleotide analysis by CE. The appearance of a leading peak at about 29min makes me unsure of whether it covers other peaks. So my question is:1. What makes a leading peak in...
26 April 2016 8,194 2 View
Why serum sample inhibit PCR amplification while this sample has no any additive to have such effect?
25 April 2016 4,634 3 View
I am measuring ROS production with DCFDA and using rotenone as my positive control. However, I can not find a concentration that will inhibit ROS production and the literature gives a very wide range.
25 April 2016 1,462 3 View
Hello, I conserved the extracted microbial DNA from kit in -20°C (in TE BUFFER), after that i was obliged to transfer the microbial DNA to + 4°C because i can't ensure the transport from a place...
25 April 2016 7,409 3 View
My protocol says to grow the bacteria at 12 degrees Celsius for 12-16 hours with vigorous shaking. I understand that the shaking allows for increased access of oxygen and nutrients for the cells....
24 April 2016 5,483 5 View
it is monoplex not multiplex and not RFLP
23 April 2016 5,278 19 View
Trying to sub clone a 300 bp gene in pET11a vector with Nde1 and BamH1 R.E . I'm getting a positive colony PCR and Re-PCR however on double digestion with the respective enzymes no fall off is...
23 April 2016 7,445 2 View
I amplified pUC-19 vector at minimum quantity of 25 Nano gram but failed to get amplification through crude DNA of Plant infected with Gemini virus. I verified the infection of virus in plant...
22 April 2016 5,659 2 View
I performed an RNA extraction using the Qiagen RNEasy Micro Kit, and my total RNA yield was lower than expected given the amount of input material. I'm considering whether to move forward with...
22 April 2016 1,320 1 View
Note: we have put a comb in side cathode, What is the reason for DNA electrophoresis in this picture?
22 April 2016 2,326 1 View
I performed qPCR on frozen rat pancreatic samples and would like to know if I have normalized my data correctly. I used two house keeping genes i.e. Actin-B and Gapdh and calculated as follows:...
22 April 2016 8,640 3 View
Hi everyone, recently I am too much worry about my RNA which is always bad in sense of genomic DNA or very big problem is degradation of RNA. I try many times, I always get high concentrated RNA...
22 April 2016 2,846 3 View
I am trying to pull down a protein with its RNA binding partners using antibody-coupled Dynabeads after UV-crosslinking. However, I am using IgG coupled beads as a negative control and getting...
21 April 2016 8,394 2 View
I have searched for references and did not find any most references are talking about the ratio of the parasite to the media without mention of pH
21 April 2016 7,919 3 View
I am trying to perform a gene knockout using pCRISPR/pCAS9 plasmids. Do I need to insert guide into pCAS9 plasmid for this purpose or just transforming the pCAS9 into the organism will be adequate?
21 April 2016 5,984 8 View
During transfer of spheroids they usually get stick on the microtip surface. how to prevent it.
21 April 2016 6,388 5 View