My lab needs to grow several strictly anaerobic bacteria that are of Biosafety Level 2. We need to use the Hungate technique (example: https://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/Kultivierungshinweise_neu_CD/engl_Anaerob_update.pdf) to keep the culture tubes under anaerobic conditions, because we do not have access to a proper anaerobic chamber. We also prefer using a biosafety cabinet to prevent handler from contacting infectious aerosols. Since the Hungate technique requires using nitrogen gas and flame to create sterile and anaerobic environment, does anyone know how to implement that inside a biosafety cabinet? Or is there any alternative to suit the purpose?