1. Determination of amino acids?
2. Determination of soluble Sugars and Protein?
4. Determination of antioxidant enzymes (CAT, POD and SOD) and synthases (PPO and PAL)?
5. Determination of MDA and H2O2 concentration?
Kindly check this
Biochemical Analysis of Rice - UKDiss.com
Rice (Oryza sativa (2n = 24) is a monocot plant and belongs to the ... Biochemical markers for analysis of diversity.
https://ukdiss.com/examples/major-source-of-macro-and-micronutrients.php
Physiological, biochemical, and molecular screening of selected ...
For physiological screening changes in plant height and ... of primer sequences of the selected SSR loci markers in rice ...
https://link.springer.com/article/10.1186/s42269-019-0087-9
Biochemical and Anatomical Changes and Yield Reduction in ...
https://www.hindawi.com/journals/bmri/2014/208584/
Anoop Kumar Srivastava dear sir please check the protocol for SOD
SOD activity was determined by measuring its ability to inhibit the photochemical reduction of Nitrobluetetrazoliumchloride (NBT), as described by (Giannopolitis and Ries, 1977). Reagents:
1. Enzyme extract: Leaf sample of 0.2 g was homogenized in mortar & pestle with 4 ml of ice cold extraction buffer (100mM phosphate buffer ofpH 7.0). Filter through muslin cloth and cernrifuge at 16,000 rpm for 15 min and supernatant was used as enzyme extract.
2. Potassium phosphate buffer: Buffer was prepared by dissolving 1.644 g KH2PO4 (acid solution) in 100 ml distt. Water and 1.008 g K2HPO4 (base solution) in 100 ml distt. Water. Then, a buffer solution was prepared by the mixing of these two solutions. The buffer solution was maintained to pH 7.0 by the help of above mentioned acid and base solutions.
3. Take 1.5 ml reaction mixture contain:- 50 mM phosphate buffer (pH 7.8)
0.1 µM EDTA
13 mM methionine
75 µM NBT
2 µM riboflavin
Take 1.5 ml reaction mixture with 50 µl enzyme extract in the tubes. Tubes are shaken well and illuminated with two 20 watt fluorescent tubes. The reaction was allowed to proceed for 15 min. after which the lights were switched off and the tubes covered with a black cloth.
Absorbance of the reaction mixture was recorded at 560 nm. One unit of SOD activity (U) was defined as the amount of enzyme required to cause 50% inhibition reduction of nitrobluetetrazolium chloride (NBT), as described by (Giannopolitis and Ries, 1977).
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