Hi everyone,

I have been trying to homogenize rat prostate hyperplastic tissues for western blotting.

1) I have tried probe homogenization using pulses on ice. It took many pulses and I still had chunks of resistant tissues.

2) I also tried a glass teflon homogenizer, which just flattened the tissue and only produced marginal effect.

3) I finally tried liquid nitrogen grinding using a mortar and a pestle. Most of the tissue grinds into a powder but I also end up with resistant chunks that do not break up. The procedure also takes me over 30 minutes per prostate with many rounds of pouring of liquid nitrogen.

4) I would have loved to try a beads homogenizer but I don't have access to one.

I only ever worked with brain in my life so I never realized how frustrating it is to work with other tissues.

I would appreciate any advice.

Best,

Mariam