Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS medium. I couldn’t expand them at all. I again got the same cell line from a company and started growing. They were doing well until the first passage. When I split the cells, they again starting to die. As per the company recommendation I used DMEM- w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate + 10% FBS but that media did not workout well for the cells and I replaced with DMEM+ 2% FBS. Is there anything else I need to be cautious while handling this cell line? Can someone suggest on optimal growth media and conditions to expand and use this cell line for assays.
Thanks in advance.