What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

What condition required for coating of Fe3O4@SiO2 by using APTES?

I tried coating Fe3O4@ SiO2 by using APTES in 5% water-ethanol but it is not coated. Kindly suggest a solution for it. Thanks

24 February 2021 5,992 4 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

Is it possible to design digital all pass filter for specific frequency range of the system?

I would like to know, Is it possible to design digital all pass filter for specific frequency range of the system to alter the phase of the system in that specific range of frequency? If yes how...

26 November 2020 1,402 5 View

In some CP patch design the polarization changes for LHCP to RHCP, What should be the possible reasons?

I have designed a dual print dual cp antenna, identical patch on both side of the substrate (placed side by side), on analysis, it is found it is showing LHCP in one band and RHCP in another band....

04 August 2020 5,136 3 View

In dual band antenna why the gain in upper band decreases?

In some printed antenna designs, generally, the gain in the upper band is larger than the lower band. However, in some cases (in my design having printed circle on both side of the substrate like...

03 August 2020 5,802 5 View

How to cap the maximum amount of reinforcement addition to make a polymer to be thermally conductive?

Addition of reinforcement with very high thermal conductivity (ex. graphene) would enhance the thermal conductive value of the developed polymer composites. How would one cap the maximum weight...

21 June 2020 9,303 11 View

We are facing rotten problem in kesar mango near mouth of it. What may be reason for that?

We are doing natural farming of kesar mango. This year we are facing rotten problem in mango near mouth of it. Last year it seems good. For this reason we are facing problem in selling if it....

02 June 2020 5,277 7 View

In COVID-19 modeling estimates for India by CDDEP, Johns Hopkins, and Princeton, what decideds peaking and decay?

COVID-19 modeling estimates for India have been presented by a team of researchers affiliated with CDDEP, Johns Hopkins, and Princeton. The results described in...

28 March 2020 1,623 2 View

Up to what temperatures can nCovid-19, novel corona virus survive?

The question does not require much explanation. Interested to know if there is some study on overall pattern of temperature range of permanent inactivity of various viruses and bacteria of all...

10 March 2020 8,749 44 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Can anyone help me with doing resorption pit assay on biomaterials for 28 days?

I culture RAW 264.7 cells on my samples and differentiate them to osteoclasts with RANKL. I want to do resorption pit assay by culturing cells for 28 days. My problem is that the macrophages keep...

01 February 2021 6,746 2 View

What M1 and M2 markers can I use for flow cytometry ?

Hi, I'm a student and I'm doing an internship. I need to asses the M1 and M2 populations of a culture using flow cytometry. From my researches, I know that this is subject to some controversy, as...

26 January 2021 299 3 View

What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

What are the validation parameters for RAW BLUE macrophages 264.7?

Currently, I am working with RAW BLUE cell lines to check the SEAP expression on addition of TLR ligands? Method developed for different TLR ligands. How experiments can design for validations?...

24 January 2021 589 2 View

Can I use 24 wells black tissue culture uncoated plate for cultuing macrophage (THP-1 treated with PMA) ?or I have to coat them?

Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...

05 January 2021 4,214 3 View

For RNA seq study and data analysis, How many tests (e.g. duplicate or triplicate) should be considered for each sample?

As we are doing RNA seq for some cell lines before and after treatment, how many tests should we do for each sample to validate data statistically?

04 January 2021 6,436 2 View

No title

Hi, I am interested to see the effect of BCG on macrophages (microglia )Therefore , I would like to know the amounts and the routes of administration which is safe in mice and how long is...

20 December 2020 7,166 2 View