DNA Ladders - Cheapest Option?

Dear Fellow Scientists, My lab just ran out of DNA ladders (and my student only told me today). The ordering process takes a long time in Sri Lanka and I'm wondering what would be the best bet for...

16 March 2017 3,543 3 View

Can anyone reckon the best user friendly software for qualitative data analysis?

I am conducting semi-structured interviews, field observations and focused group discussions for my study. I would like to know the most suitable, user-friendly and convenient software for such...

13 November 2016 4,034 17 View

How to interpret three way ANOVA significant interaction effect (none of the others are significant) ?

I conducted 2*2*2 experiment and only main interaction is significant (2*2*2). All the other interactions and direct effects are not significant. Please let me know the steps of interpreting the...

12 May 2016 2,260 4 View

Can anyone suggest some universal primers for Nitrogen Fixers?

Hi All, We have been using the PolF and PolR primers (Poly et al., 2001) to identify nitrogen fixers but unfortunately only ~ 10% of the isolated bacteria are giving the correct 360 bp...

08 September 2015 1,478 3 View

Does anyone know about Taq polymerase storing temperature?

Hi, We don't have with us a -20C freezer and we are storing Taq polymerase at -80C........Would this affect the lifetime of the Taq Polymerase ? Your input would be greatly appreciated. Best...

02 September 2015 7,138 6 View

How to convert .GEL extension files to image files?

I have some old gel scan files I need to convert to image files - converting .GEL extensions to image formats. Is there a free software that is available for me to convert these files to a graphic...

17 September 2014 7,922 5 View

Human versus Plant Dcp2 mRNA Decapping Enzyme papers

Dear All, I just looked at the human hDCP2 enzyme paper from 2002, that has a staggering 439 citations, while mine on the AtDCP2 enzyme, published in 2007, in Nucleic Acids Research, has 25....

01 January 1970 5,640 1 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

How to perform EEG classification of Alzheimer and control groups?

I am doing a project on classification of EEG signals of Alzheimer's disease and control groups. Previously, I extracted the alpha power of the EEG signals and found out the strengths. I want a...

18 February 2021 8,502 2 View

How to perform Kaplan-meier survival analysis in SPSS?

Hi everyone, This is my first time of attempting to run survival analysis. I have a data on patients with End-stage Kidney Diseases (ESKD). I would want to run survival probability by treatment...

16 February 2021 2,647 3 View

Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Why am I getting such large heats of dilution in my Isothermal Titration Calorimetry Experiment?

I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...

07 February 2021 7,532 1 View

Patients with chronic kidney disease could get COVID-19 vaccine?

04 February 2021 278 4 View

Need a research paper for multi speaker and single microphone acoustic echo cancellation?

Hi, I am working on a problem that involves multiple smartphones/tablets. All devices are playing the same sound with synchronization difference up to +- 5ms. Now, there is an echo issue and I...

02 February 2021 5,929 3 View

How can you accurately induce calcium overload and measure membrane potential in cultured Tg2576 neuronal cells using whole cell patch clamp method?

Hi there, I'm currently working on a project where i need to design an experiment but as i have little to no experience with this level of experimental design and no face to face help i am really...

22 January 2021 6,728 3 View

Where can I get a Music therapy database for Alzheimer's Disease?

I am working on the effect of music therapy on persons with Alzheimer's Disease. I require EEG signals of healthy individuals and people with Alzheimer's Disease/Dementia that have undergone music...

22 January 2021 8,613 5 View