CRISPR-Cas9 Efficiency: "What are the most effective strategies to improve the specificity and reduce off-target effects of CRISPR-Cas9 in mammalian ?

Harsha Siva Shesha Sai @Harsha-Siva-Shesha-Sai

20 August 2024 1 9K Report

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Harsha Siva Shesha Sai

High-Fidelity Cas9 Variants: Engineering Cas9 proteins, such as SpCas9-HF1, eSpCas9, and HypaCas9, that have been modified to reduce non-specific DNA interactions can decrease off-target effects while maintaining on-target efficiency.
Base Editors: Using base editors (e.g., cytosine base editors or adenine base editors) that convert single nucleotides without inducing double-strand breaks can enhance specificity and reduce off-target effects.

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