Hi everyone, a while ago, I received some antibodies that were not stored refrigerated properly. I would like to know if there is any way to check if they are working properly.
Do you have any tips on how I can do this?
Most of these antibodies are for immune cells, and others are melanoma markers.
Thank you very much for your help!!
All the best!
Hello Jean,
Yes, there is a way out.
To confirm that your antibodies are still functional, you may test them on positive controls.
Best Wishes.
The stability of antibodies depends on factors such as storage conditions, duration of exposure to suboptimal temperatures, and the specific antibody type (monoclonal vs. polyclonal). To assess their functionality:
If signal strength is weak or background noise is high, the antibody may have degraded. If results are comparable to a properly stored batch, the antibodies are likely functional.