Hi everyone! I am trying to harvest Bronchial and mediastinal lymph nodes from mouse. But I am not sure how to do so. Is there any video and protocol I can follow? Thanks!
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?
06 August 2024 2,508 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds
04 August 2024 3,019 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...
05 August 2024 8,056 1 View
Hi all, I am working on a project which needs expansion of CD45+ selected cells from tumor and lymph nodes. My Tumor samples expand good but my samples from the lymph nodes are less and less...
23 May 2024 2,839 0 View
Aspiration of swollen lymph node from different swollen regions brought blood instead of lymph node aspirate.
05 April 2024 7,671 0 View
I need to make a lysing solution to lyse the RBC and WBC for using in cell counter of sysmex k1000. When I use the blood control all parameter are normal but when I used the human blood the cell...
11 February 2024 8,536 0 View
As we know, lymph nodes have a mixed population of cells, how do we ensure only B cells are getting isolated and subsequently fused with myeloma cells? Thanks in advance!
30 January 2024 3,449 2 View
Hello to all of the researchers, i need to isolate and grow T lymphocytes from swine lymph nodes. I need you people to guide/help me or send me any protocol/video for T cells isolation from wine...
24 January 2024 1,380 1 View
According to guidelines.
02 October 2023 4,684 0 View
Hi! I just had a manuscript rejected for publication, reviewer 2 stated that since we did not perform repeats, just replicates, the reproducibility of the results is compromised. Basically, our...
21 July 2023 5,834 0 View
How do the immune changes of intestinal lamina propria and mesenteric lymph nodes affect the immune changes of spleen? Can intestinal lymphocytes migrate to the spleen?
07 July 2023 5,699 1 View
How many naïve T cells we can get from a C57BL/6J mouse spleen and lymph nodes? or in other word, what percentage of the cells from spleen and lymph nodes are naïve T cells?
15 May 2023 1,478 3 View