Contact experts in Liquid Chromatography to get answers
3,959 views 348 posts
Questions related to Liquid Chromatography
Hello, does anyone have any experience with packing columns for nano LC? Any suggestions on the tubings and the method used for column packing?
05 November 2014 1,340 3 View
In AOAC 2009.01 and AOAC 2011.25, liquid chromatography-refractive index is used for measuring low molecular soluble dietary fiber. Can I use ion chromatography to measure low molecular soluble...
03 November 2014 329 4 View
Am undergoing an MSc in Clinical biochemistry at Middlesex University
10 October 2014 638 2 View
Does someone have experience using methyl orange to monitor silylation by MSTFA? I found an old article by Donike (Journal of Chromatography, 115 (1975) 591-595) proposing the addition of a small...
10 October 2014 4,088 0 View
On the multi-mycotoxin determination studies with LC-MS/MS, if when we already find out the validation (LOD/LOQ, recovery, precision) and sample results with the total LC-MS/MS run time of 25min....
09 September 2014 1,490 12 View
The monolith prepared is HILIC
09 September 2014 1,967 2 View
This publication uses a column 150 x 4.6 mm, the void volume of this column is 1.75 mL. At the flow rate of 1.2 mL/min, the non-retain peak should be out at 1.75/1.2 = 1.45 min. However, the...
09 September 2014 2,750 11 View
I am doing some LC-MS analysis and I got well resolved peaks in the chromatogram with the UV-detector. However, the MS detector shows very poor resolution. How can I improve this?
09 September 2014 3,176 10 View
09 September 2014 246 2 View
09 September 2014 9,081 10 View
I have isolated compounds from a plant extract by silica gel chromatography. I would like to further characterize it with LC-MS. What is the minimum amount of sample required for LC-MS. Can...
30 July 2014 5,231 16 View
Is there any LC-UV analytical method available for quantitative determination of Trypsin and/or Chymotrypsin?
19 July 2014 3,206 1 View
Commercial nano LC columns (a common choice in proteomics) may feature e.g. "regular" 3 um C18 attached particles, UHPLC particles, fused core particles, silica monoliths, organic monoliths. Some...
07 July 2014 8,265 35 View
07 July 2014 5,354 1 View
Practical method
07 July 2014 8,144 4 View
Separating sugars on a Hypercarb porous graphitic carbon column, retention time shift between runs is unacceptable. (Using MeOH/H2O+HCOOH eluent) Any suggestion for a good washing...
04 July 2014 4,907 3 View
I have an analyte which absorbs at 196 nm on PDA detector. So is it possible to shift its absorption between 200-300 nm by combination of mobile phase, buffer or modifier? how ?
20 June 2014 5,087 12 View
I have been analysing compounds with a GCMS for over few years. Some of them I would like to test for biological activity. I have attempted liquid separation by several means with no success to...
06 June 2014 8,515 5 View
I am working on two plants for my PhD and I want to know if GC be used as final tool for identification?
06 June 2014 4,703 10 View
06 June 2014 8,737 13 View
I am developing new methods to determine hidrophilic compounds and I want to introduce a new method of separation based on hidrophilic interaction liquid chromatography in my thesis. This is a...
23 May 2014 7,742 3 View
Especially in temperature programming. Recently I am screening extracts of algae and looking for pharmaceuticals, using GC-MS to analyse the sample. What is the best condition I should use?
18 May 2014 4,904 2 View
I am facing a problem to get a positive enzymatic reaction of ACE as a catalyst to hydrolyze a substrate (HHL) which liberates Hippuric acid (HA) and Hip-Leu (HL). To be clear on the research...
08 May 2014 7,197 3 View
05 May 2014 2,905 4 View