Purity on nanodrop for the plasmid isolated?

I isolated a plasmid (image attached along with), however the 260/230 ratio was less than desired i.e. 1.29 as compared to the ideal 2.0-2.2. Would it influence the digestion reaction?

09 November 2020 7,666 2 View

Test to apply on likert scale data. I want to test the impact of e-banking on bank performance. which test should I apply?

23 October 2020 4,590 9 View

I want to buy Human Squamous Cell Carcinoma Cell Lines for my phD research .?

i want to buy Human Squamous Cell Carcinoma Cell Lines for my PhD research .? One place is NCCS Pune...Can some give more suggestions..? Humble Regards

20 September 2020 3,940 3 View

What is the best buffer for plants to avoid osmotic shock?

Hi! I am doing an experiment where I need to spray a chemical on plant. I will be using autoclaved milliQ water as a base to dissolve small quantities of that chemical. I was wondering how can we...

09 April 2020 5,808 2 View

Which remediation method is most suitable for heavy metals remediation from contaminated soil and groundwater nearby industrial and mining area?

Suggest me some effective and less time taking methods for remediation purpose and also economically feasible.

02 April 2019 3,186 12 View

I am interested in knowing how to do the scoring/analysis of a sentence completion task with respect to a linguistic research question?

Is it like you provide a certain score to the different answers expected for each sentence fragment and then calculate the score later based on the response given by the participants. Please...

31 July 2017 6,537 7 View

Which software is best for Degenerate primers designing?

11 February 2016 3,913 3 View

Is there any human motor neuronal cell line available?

We are interested in examining the effect of CSF from MND patients on motor neuron cells.

29 March 2015 4,112 1 View

Kindly let me know what's the best way to qualitatively and quantitatively identify Menthol in a herbal formulation (herbal tea in granular form)?

Should it be GC-MS? Also, if we are testing same formulation's sample few months apart, menthol being volatile is liable to decrease with time? Yes / No How to maintain the stability of menthol in...

27 January 2015 1,687 6 View

Can anyone suggest the 'Soak & degassing Time' & 'Temperature' for BET analysis of some porous Copper pellets?

Sample details are as follows - 1) Porous Copper pellet 2) Porous 'Copper+graphene' pellet 3) Porous 'Copper+CNT' pellet 4) Porous 'Copper+diamond' pellet

18 November 2014 3,332 10 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View