Is there any LC-UV analytical method available for quantitative determination of Trypsin and/or Chymotrypsin?
Sir I need to quantitate the content of trypsin in a mixture of composition of trypsin-chymotrypsin powder. The activity ca be determined through UV-vis spectrophotometer kinetics experiment.
1. What to do when there is something you don’t understand? 2. Do you have any tips you’d like to share? 3. What parts of title can tell us about the whole write up? 4. How to decide which part of...
02 March 2016 1,821 2 View
What is possible legitimacy of publishing in such a way. Does it have any same value as that published in Journals or is it just to show that one has done some work ahead of any other in that...
01 February 2016 3,932 4 View
I have the problem of appearance of nan-specific bands when I change the volume of DMSO, Pfu, Polymerase buffer and or DW in sample solution for PCR. I also change the annealing temperature due to...
09 October 2015 2,911 5 View
What are its basic parts and their role in the process of water treatment?What does the word "Hybrid" stand for?
08 September 2014 1,521 7 View
Talking with colleagues, it seems that the possible usefulness of an HPLC method development software repeatedly comes into the analyst's mind during the development of a tough HPLC/UPLC assay....
03 April 2014 6,680 1 View
What would be the best ratio of AgNO3:PVP+PEG, so as to have complete reduction of my precursor and also get 100% capped NPs?
31 December 2013 2,802 2 View
How can I get the double divisor ratio spectra method for ternary components analysis, while using double beam 1650PC Shimadzu spectrophotometer, I can not understand the methods produced in few...
31 December 2013 2,779 1 View
I have developed Silver NPs with PEG-PVP combinations but sometimes I get various coloration changes, I do not understand which colour is the endpoint of my reaction between precursor and reducing...
31 December 2013 10,015 8 View
In forced degradation studies for API/drug product we are performing hydrolysis (acid, base & water), Oxidation, Photolysis and thermal degradation conditions. But why we are not performing reduction.
31 December 2013 9,355 5 View
PEG 4000, PEG 6000, PEG35000 and PVP25000, PVP30000 are available for my work. Which ones will be better? Why ?
11 December 2013 7,227 3 View
I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...
12 August 2024 862 0 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
Please go through my Abstract. I can also share a proposed Thesis Outline.
04 August 2024 2,077 0 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View