Separating sugars on a Hypercarb porous graphitic carbon column, retention time shift between runs is unacceptable. (Using MeOH/H2O+HCOOH eluent)
Any suggestion for a good washing procedure?
Thanks a lot!
Dear Farooq Wahab,
Thank you for your thorough answer!
bests,
Szabolcs
it takes more time for stabilization of this column then RP column. I suggest you run 5 to 10 blk before sequence.
Two features of Hypercarb must be considered when using it for applications involving carbohydrates and anionic analytes. First, Hypercarb contains weak anion exchange sites on its surface. This aspect is primarily responsible for Hypercarb carbohydrate retention and selectivity. Second, these anion exchange sites are sensitive to oxidizing and reducing agents. The symptoms suggest the latter may be your problem. Try treating your column with ascorbic acid to restore the native oxidation state of Hypercarb.
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