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Questions related to Liquid Chromatography
I planning to ask my supervisor to buy a flash column system, CombiFlash is preferred, well, just planning currently, but I can't find price information online, any guys help me out please.
04 April 2016 6,886 1 View
For some unknown reasons, the software (Empower 2) doesn't provide mass distribution values for my samples. It integrates the chromatogram and detects all the peaks/retention times very well but...
01 April 2016 1,791 2 View
I was synthesized a pyrazoline derivative, and while TLC, the spot was always blue and yellow that stuck together. Although i TLC it with various solvent, they were not separate.
18 March 2016 291 5 View
Dear all, I have been working to troubleshoot my LC-MS/MS/MS experiments for several months already. There seems to be multiple peaks of the same product ion observed on the LC-MS chromatogram but...
11 March 2016 5,655 13 View
It is (1+) and strongly present with high ACN content at the end of our LC gradients (Setup: Ultimate 3000RSLCnano HPLC coupled to an impactII MS running plant proteomics samples). Didn't find any...
10 March 2016 5,301 6 View
It is a chromatogram of phenolics compounds (standard compounds) and with a red arrow I point the place where a high peak (Peak 1) appears when I inject a methanolic extract from black mulberry...
03 March 2016 9,180 3 View
03 March 2016 8,914 6 View
Hi. I am trying to develop a new analytical method by LC. Mi matrix is intraperitoneal fluid. I´ve read some articles and for the development of the method validation some authors use water as a...
03 March 2016 4,254 3 View
How can i prevent peak fronting of acidic compound in TLC?
03 March 2016 9,856 4 View
03 March 2016 7,822 15 View
I'm currently having to problems with our Agilent LC/MS, my first problem is that the pressure becomes a bit high when I switch from 100% ACN to 50% water and 50% ACN. The max bar I usually see...
03 March 2016 2,202 6 View
Hi everyone, we are having an issue with our Dionex ICS 5000. We are analysis phenol levels in alcohol samples from different distilleries within the company using HILIC and electrochemical...
03 March 2016 7,186 11 View
There is no given protocol in GE's manual for regeneration of HiTrap Desalting column. So I wonder, is there anybody who reuses this column? How to do that?
03 March 2016 4,232 3 View
Read 4 answers by scientists to the question asked by Velusamy Arumugam on Feb 18, 2016
18 February 2016 7,110 2 View
I'm trying to run a protein purification experiment using liquid chromatography. However, I forgot to remove DNA and other cellular debris from cell sonification before adding resin. What should I...
05 February 2016 534 2 View
Please suggest any HPLC method for Boronic acid samples with good retention and peak shape. Thanks -Bharani
03 February 2016 4,430 2 View
there are many protocol with this drug so, if any one have a hint of way to pick one methodology I will appreciate.
02 February 2016 2,588 4 View
I am trying to isolate lipoprotein A1 by running liquid chromatography on Sephacryl S200 packed column, 5x150cm. The buffer (moving phase) goes thru the top via gravity, and on the bottom i have a...
02 February 2016 5,524 3 View
we are analysing various ganoderic acids in Ganoderma Mushroom. What is the best labelled standard that i can use to calculate the recovery and EE of my method.
02 February 2016 9,847 1 View
Read 4 answers by scientists with 3 recommendations from their colleagues to the question asked by Velusamy Arumugam on Feb 18, 2016
02 February 2016 10,044 3 View
we are using the column Kinetex C18 column (2.6 x 150 mm i.d., 1.7 µm particle size.and changing the proportion of the mobile phase (acetonitrile and water) no big change was observed.
02 February 2016 9,959 6 View
How are FTIR spectrometer and Q-TOF LC/MS differentially capable in identifying unknown compounds ?
02 February 2016 8,183 2 View
recently after few sets of sample and standard injection, the sequence stops randomly with the error message stating power failure on DAD icon with still power supply from ups. if anyone can...
02 February 2016 3,335 14 View
01 February 2016 2,775 1 View