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Questions related to Liquid Chromatography
I am developing the method for simultaneous detection of gallic acid, pyrogallol, ethyl gallate, catechin,epicatechin and quercetin in a plant extract through Reverse Phase Ultra Flow Liquid...
29 May 2018 8,392 3 View
In liquid chromatography or gas chromatography we can tell the plate height is the distance between two equilibration steps. But we do not have any stationary phase in CE. So how we explain what...
04 April 2018 1,629 0 View
Has anyone done LCMS on condition media for protein quantification? I have this media (after the exposure of different flow conditions) that i would like to use LCMS for protein quantification...
13 February 2018 2,571 6 View
Have been facing problem related to the column pressure from quite some time. Started the column clean-up procedure using 0.5M NaOH. Firstly, washed it twice the column volume with milliQ. Then,...
08 February 2018 7,098 9 View
Can someone please share the information or experience on flushing the anion exchange column from the arsenic speciation kit by ESI? The manufacturer doesn't provide any information about this...
21 November 2017 1,136 3 View
What is the purpose of using Methanol in doing Liquid Chromatography Mass Spectrometry?
11 November 2017 2,102 0 View
Hi Mycotoxicologists, I am trying to find out if the Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS) is an approved or the best method for detection and analyses of MULTI-MYCOTOXIN in...
10 October 2017 8,372 0 View
Hello, Can anyone advise me where to find an example(s) of impurities profiling data (.raw files) acquired by liquid chromatography/mass spectrometry? Ideally, I would like to have several stages...
09 September 2017 9,448 3 View
My peaks have a slight peak tailing so half height or half width is appropriate but do I use R = (RT1 - RT2) / [0.5 * (W1 + W2)],...
26 August 2017 1,697 4 View
I want to try basic conditions for LCMS in negative mode to try to satisfy the different pKa values of my 5 analytes which gear towards acidic and basic. pH5 is fine but the basic analytes can...
08 August 2017 1,564 5 View
I am currently studying Individual and joint effect of PAHs and surfactants on CYP 1A enzymes in earthworms, looking at the EROD activity using the UPLC. My current mobile phase is...
08 August 2017 2,658 8 View
Are all the peaks read of from Liquid chromatography–Mass spectrometry (LC-MS) always relevant. If yes, how will you analyse each of them assuming they appeared in 100's?
08 August 2017 4,740 6 View
05 August 2017 675 2 View
How are they related to anthraquinone, anthracine.
05 August 2017 6,338 1 View
Hello Folks, I am planning to measure the endogenous compounds (e.g.retinoids) in liver tissue with LC-MS/MS. But it is very hard to get a control matrix sample for method development and...
12 July 2017 3,671 4 View
Hi All, Am trying to quantify benzalkonium chloride by LCMSMS. I have optimised the method got the parent and daughter ion. Have injected the standard, peak was eluting. After that injected blank...
07 July 2017 9,643 2 View
Hello, I'm trying to upgrade to Analyst 1.6.3 so I can get off Windows XP and onto Windows 7. However, DCMS link version 2.1 is not working. It loads initially but then is not showing up in...
07 July 2017 6,023 3 View
07 July 2017 6,124 5 View
I have a new molecule for which I have initially developed an isocratic LC-MS/MS method (Synergi Fusion column, H2O/ACN 20/80 (+0.1% HCOOH)). Analysis of this method gives limited sensitivity...
26 June 2017 1,879 11 View
06 June 2017 1,207 12 View
I am working with HPLC Shimadzu 20AD fitted with LC20AD. The pressure does not rise
05 May 2017 9,265 11 View
Dear All, I try to measure the concentration of melatonin in blood samples. For this reason I use the following extraction method: First, I spin down the blood cells by CF at 1200 rcf / 5 min....
05 May 2017 1,090 1 View
Hello, I try to develop a method for determination of PITC derivatized amino acids in cockroach but immediately i established my conditions for the separation (amino acids standard) which gives...
05 May 2017 5,516 8 View
I am looking for a more in-depth comparison in between these techniques with regards to the general analysis of compounds, if anyone could help? Off the top of my head, pros of the qNMR would be...
04 April 2017 4,841 4 View