Am undergoing an MSc in Clinical biochemistry at Middlesex University
Hello, I would recommend you to go through the attached link and have some idea (related literature) first. Good Luck!
http://www.dionex.com/en-us/markets/life-science/protein-sciences/amino-acid-analysis/lp-71622.html
There are plenty of papers on AA separation. Please check the literatura and refine your question
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