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Questions related to Liquid Chromatography
In NLC system in which temperature this system would release its contents?
23 January 2016 2,269 1 View
atomic absorption spectrometry
01 January 2016 6,516 3 View
01 January 2016 1,619 1 View
(UP)LC-MS/MS is a chemistry technique that combines the physical separation capabilities of liquid chromatography with the mass analysis capabilities of mass spectrometry.
01 January 2016 1,311 0 View
Hello, everyone, I have trouble in my method of reversed phase conditions (C18) of HPLC, In my method, the mobile phase consists of water and Acetonitrile Containing 0.1% TFA in gradient method...
28 December 2015 8,676 12 View
12 December 2015 5,542 13 View
peptides eluted in 1%formic acid /60 % acetonitrile. i have tried to partially quantify the peptides at 280 nm in a nanodrop. the reading was about 2.3 ug/ul in 6ul but with out any peak. should i...
12 December 2015 2,227 2 View
11 December 2015 623 2 View
Hello. I am performing a catalytic reaction. I need to separate alcohols (e.g. ethanol, methanol and so on), glycols (ethylene glycol, 1,3 propylene glycol and so on), and some other organic...
26 October 2015 3,091 8 View
Through the web I could find only 6 to 10 stationary phases like C1 to C18 and cyano amino diol etc. I opt to get a detailed list. Please suggest a reference.
10 October 2015 2,205 10 View
08 October 2015 9,470 8 View
Hi everybody! I could really use a list of endogenous human metabolites with traits (most interested in hydrophobicity, charge and Mw). Does anybody know if this exits? I want to use it to attempt...
16 September 2015 7,483 3 View
What is the difference between pre-treated with acetonitrile and directly used real sample analysis for measurement?
08 August 2015 5,464 2 View
I would like to ask some more questions about a problem I described previously on this forum. I started working on the Acquity Xevo TQD system more than a year ago in April 2014. Immediately it...
08 August 2015 704 11 View
Hi everybody, We recently plan to buy a LC-MS for large-scale metabolism in plant, e.g. citrus, tomato. The basic flowchart is identification of metabolites using QTOF (Agilent 6420B in our...
07 July 2015 7,327 8 View
Hi there, I'm trying to separate these compounds (urea, creatine and creatinine) from a mixture of peptides. I'm wondering whether these compounds will bind to the column, too? I'm hoping they...
30 May 2015 4,070 9 View
In analysis of a weak acid with pKa of 4.6, using mobile phase of phosphate buffer pH 7.7 and methanol (63:37, v/v) and hypersil C18 column, the retention time of this analyte is not stable (it is...
21 May 2015 8,194 13 View
05 May 2015 318 14 View
Autosampler loop volume is 50 microliter. When I mix my sample with the buffer by autosampler ( using insert or not), fifty fifty, the peak areas are never reaching the expected value. When I mix...
05 May 2015 4,776 6 View
05 May 2015 5,856 10 View
My mobile phase is acetonitrile and water (0,1% formic acid) and two compounds (Methotrexate and Ciprofloxacin - cytostatic drugs) show double peaks but these peaks are far from each other. eg:...
28 April 2015 6,121 12 View
04 April 2015 5,865 13 View
I have an HPLC column with an i.d. of 4.6 mm and a flux of 400 microL/min which in principle requires the ESI source to have between 60 and 100 arbitrary units of sheath gas. I intend to do...
04 April 2015 5,121 6 View
To check whether a peak is not contaminated with other compound(s) is very important for proving the selectivity of a method. We can do it easily if we use DAD or MS detectors. Generally, for...
24 March 2015 3,300 4 View