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Questions related to Liquid Chromatography
is reversed phase liquid chromatography used only in gradient elution ? what i know we use in reversed phase liquid chromatography (hydrophobic mobile phase)
10 October 2016 358 10 View
Hello Everyone, I'm working with Agilent 1220 LC system. The openlab software shows that the pump is idle but its shows the pressure is zero. It looks like pump is not building pressure. Can...
10 October 2016 9,580 9 View
Dear colleagues, I am a graduate student, I began my Ph.D. some months ago, and I am trying to work with an Eksigent nanoLC 1D plus.I would like to change the valve position for inject using...
01 October 2016 2,283 2 View
In Norway, there is a debate regarding the use of asthma medicine in sports. The debate was fueled by the doping violation of the cross-country skier Martin Sundby. It has also been stoked by the...
10 September 2016 3,025 3 View
Dear Scientists, I have a problem with (probably) plugged column(s) after use of ammonium acetate buffer. Let me explain what I did so far. On the beginning I worked on ACE Excel C18 column in...
09 September 2016 6,069 10 View
Please do you have a functional LC/MS in your Institution that i can use to analyse plasma and saliva samples? I will like to find out if you can allow me to analyze my samples using your liquid...
09 September 2016 9,519 1 View
09 September 2016 9,673 3 View
I am currently doing a school project on Analysis of Saccharides in Fruit Juice Drinks, and I have to choose a method either LC or LCMS. After reading several articles and journals, my team...
08 August 2016 7,317 0 View
Hey Experts, I am planning to buy LC detector for lipid analysis. Can you please suggest me which LC detector would be best for lipid analysis? I am using SCIEX 5500 QTRAP and Shimadzu LC. Thank...
05 August 2016 8,355 6 View
Hello, I have a problem with leaking HPLC pump (I am using Agilent 1100 with a binary pump). I noticed today that the liquid is not flowing through the column (or to the waste), but it appears on...
03 August 2016 6,387 7 View
I am start the analysis of glyphosate. I have the ISO/TC 147/SC 2 N1145, but the procedure has many steps. I am asking help. If someone already made a flow-sheet of the procedure to be more...
12 July 2016 3,250 1 View
07 July 2016 1,295 1 View
Many stationary phases and columns in NP-LC can be used in HILIC analysis. So what is the difference between the stationary phases of NP-LC and HILIC.
07 July 2016 8,765 7 View
I want to analyse concentration of lovastatin in the fermented rice straw extract.
02 June 2016 8,451 3 View
Hello. I may have a chance that get Prep HPLC(Waters delta prep, 2005 purchase), I don't exactly know this machine which detector equip or heating for column, and rate and pressure(pump). I...
26 May 2016 3,400 7 View
In order to study changes in the proteome of biological fluids, what is hte best methods, 2D-DIGE or liquid chromatography?
11 May 2016 9,629 1 View
In case of liquid chromatography when methanol is used as organic modifier it gives broad peak shape in comparison to Acetonitrile. Even though the buffer ratio may remain same for the both.
05 May 2016 1,014 1 View
For someone who's familiar with LC's: what should be the vector for the director in that case? I already familiar with the cases of splay and bend cell but couldn't understand how to derive it to...
05 May 2016 7,765 0 View
05 May 2016 4,160 1 View
According to some recent reports, several intact proteins,just like small-molecule compounds, can also be measured by LC-MS/MS with MRM mode. How to obtain and select the marker fragment(s) and...
05 May 2016 3,498 1 View
Hello... During my study sample analysis, due to some technical problem curtain gas of LCMSMS was modified to 30 instead of 10. So my question is which parameters are required to perform in...
04 April 2016 9,142 4 View
What is the function of end capped column in HPLC? How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?
04 April 2016 6,162 1 View
I planning to ask my supervisor to buy a flash column system, CombiFlash is preferred, well, just planning currently, but I can't find price information online, any guys help me out please.
04 April 2016 4,080 1 View
For some unknown reasons, the software (Empower 2) doesn't provide mass distribution values for my samples. It integrates the chromatogram and detects all the peaks/retention times very well but...
04 April 2016 2,223 1 View