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Questions related to Liquid Chromatography
Especially in temperature programming. Recently I am screening extracts of algae and looking for pharmaceuticals, using GC-MS to analyse the sample. What is the best condition I should use?
05 May 2014 736 2 View
For some time I have tryied to determine zearalenone in tissues from various parts of the turkey using HPLC-FLD. As a method of sample preparation I use LLE and as a cleanup step MIP-SPE. However,...
05 May 2014 5,598 3 View
I have isolated fractions from plant extract using column chromatography. I would like to identify the plant compounds using LC-ESI-MS. But I don't know the method for development of LC-ESI-MS.
05 May 2014 9,657 5 View
I am looking for the tools to predict peptide retention time for LC-MS.
11 April 2014 9,822 4 View
I am following the installation instructions at this website: http://lcms-sim.sourceforge.net/ However I am stuck in the 3rd instruction. When i open the makefile, it says: # You need to set...
08 April 2014 5,552 2 View
See above
04 April 2014 8,174 3 View
04 April 2014 9,576 4 View
04 April 2014 472 2 View
Chiral separation.
17 March 2014 6,547 4 View
I am attempting to derivatize polymyxins using FMOC - Cl. I run the derivatives on UPLC, but unfortunately I am struggling to determine which peaks are polymyxins. Briefly, using a vacuum...
08 March 2014 8,643 4 View
I would like to ask whether the MS library is able to identify the compounds in a crude extract? Or still have to go through purification before sending to the MS to identify?
03 March 2014 9,624 4 View
As above.
03 March 2014 2,149 7 View
What does this word mean "an aliquot of the sample"?
03 March 2014 5,563 5 View
Silica gels and bare silica
03 March 2014 4,979 5 View
I want to learn about the different methods for predicting the elution time. I also want to read some papers which provide a software. Do you have any advice?
03 March 2014 4,805 2 View
INDYK&WOOLLARD: JOURNAL OF AOAC INTERNATIONAL VOL. 83, NO. 1, 2000 123 Determination of Vitamin K in Milk and Infant Formulas by Liquid Chromatography: Collaborative Study (a) Preparation of...
03 March 2014 1,486 1 View
03 March 2014 3,728 6 View
I want information about LC/IR and LC/NMR.
03 March 2014 8,365 2 View
Dionex HPLC system using RF2000 detector
03 March 2014 4,523 2 View
What is the best column (stationary phase) for separation of polar vitamins in pharmaceutical preparations? Is it HILIC or silica?
03 March 2014 4,659 12 View
03 March 2014 4,492 5 View
In method validation (ICH and USP) they use specificity of an HPLC method. Can we say that a validated HPLC method is specific for a particular analyte (e.g. pharmaceutical or impurity)? What I...
03 March 2014 6,097 10 View
reversed-phase (RP)
03 March 2014 1,376 10 View
03 March 2014 7,628 9 View